PDH E1 Alpha Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PDH E1 Alpha Monoclonal Antibody for WB, IP, IF, FC, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human

Applications

WB, IP, IF, FC, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2B3C10

Cat no : 66119-1-PBS

Synonyms

PDH E1 Alpha, PDH E1α, PDHA1, PDHE1 A type I, pyruvate dehydrogenase E1 α subunit

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Validation Data Gallery

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  WB analysis of HEK293 cells using 66119-1-Ig (same clone as 66119-1-PBS)

  WB result of PDHA1 antibody (66119-1-Ig, 1:30000) with si-control and si-PDHA1 transfected HEK293 cells. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 66119-1-Ig (same clone as 66119-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66119-1-Ig (PDH E1α antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IP experiment of HEK-293 using 66119-1-Ig (same clone as 66119-1-PBS)

  IP result of anti-PDH E1 Alpha (IP:66119-1-Ig, 3ug; Detection:66119-1-Ig 1:10000) with HEK-293 cells lysate 1800ug. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver cancer using 66119-1-Ig (same clone as 66119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66119-1-Ig (same clone as 66119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 66119-1-Ig (same clone as 66119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 66119-1-Ig (same clone as 66119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 10x lens). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 66119-1-Ig (same clone as 66119-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66119-1-Ig (PDH E1α antibody at dilution of 1:50(under 40x lens). This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

  Immunofluorescent analysis of HepG2 cells using 66119-1-Ig (PDH E1α antibody) at dilution of 1:100 and Rhodamine-Goat anti-Mouse IgG. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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  FC experiment of HepG2 using 66119-1-Ig (same clone as 66119-1-PBS)

  1X10^6 HepG2 cells were stained with .2ug PDH E1α antibody (66119-1-Ig, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Mouse IgG(H+L) with dilution 1:1000. This data was developed using the same antibody clone with 66119-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66119-1-PBS targets PDH E1 Alpha in WB, IP, IF, FC, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDH E1 Alpha fusion protein Ag12556
• Full Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 43 kDa
• GenBank Accession Number: BC002406
• Gene Symbol: PDHA1
• Gene ID (NCBI): 5160
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PDHA1(Pyruvate dehydrogenase E1 component subunit alpha, somatic form, mitochondrial) is also named as PHE1A.It is one of the 3 enzymes of the pyruvate dehydrogenase complex which is a nuclear-encoded mitochondrial matrix multienzyme complex that provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle by catalyzing the irreversible conversion of pyruvate into acetyl-CoA(PMID:7853374).It has 4 isoforms produced by alternative splicing.Defects in PDHA1 are a cause of pyruvate dehydrogenase E1-alpha deficiency (PDHAD) and X-linked Leigh syndrome (X-LS).