PDGFRL Polyclonal antibody

PDGFRL Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 16217-1-AP

Synonyms

PRLTS, Platelet-derived growth factor receptor-like protein, PDGRL, PDGFR-like protein, PDGFR like protein

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Validation Data Gallery

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  WB analysis of L02 using 16217-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 16217-1-AP (PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 16217-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16217-1-AP (PDGFRL Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 16217-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse liver using 16217-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse liver using 16217-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 16217-1-AP (PDGFRL Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of L02 using 16217-1-AP

  Immunofluorescent analysis of L02 cells using 16217-1-AP (PDGFRL antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: L02 cells, mouse liver tissue
• Positive IHC detected in: human liver cancer tissue, mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

16217-1-AP targets PDGFRL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDGFRL fusion protein Ag9218
• Full Name: platelet-derived growth factor receptor-like
• Calculated Molecular Weight: 375 aa, 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC010927
• Gene Symbol: PDGFRL
• Gene ID (NCBI): 5157
• RRID: AB_2878230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nat Commun; A single cell atlas of frozen shoulder capsule identifies features associated with inflammatory fibrosis resolution; Authors - Michael T H Ng
human	WB	Aging (Albany NY); Identification of a unique stress response state of T cells-related gene signature in patients with gastric cancer; Authors - Qin Yang ; KD Validated
human	IHC	Mol Carcinog; The cumulative antitumor effects of regorafenib and radiotherapy in hepatocellular carcinoma; Authors - Shuwen Kuang