PDGFR alpha Monoclonal antibody

PDGFR alpha Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

4

CloneNo.

2C11B8

Reviews (1)

Cat no : 60045-1-Ig

Synonyms

CD140A, CD140a antigen, PDGF R alpha, PDGF receptor alpha, PDGFR2, PDGFRA, PDGFRα, PDGFR-α, Rhe PDGFRA

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Validation Data Gallery

• 

  WB analysis of THP-1 using 60045-1-Ig

  THP-1 cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 60045-1-Ig

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-251 using 60045-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HUVEC using 60045-1-Ig

  HUVEC cells were subjected to SDS PAGE followed by western blot with 60045-1-Ig (PDGFRA Antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: THP-1 cells, HT-1080 cells, HUVEC cells, U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:20000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 3 publications below

Product Information

60045-1-Ig targets PDGFR alpha in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDGFR alpha fusion protein Ag1385
• Full Name: platelet-derived growth factor receptor, alpha polypeptide
• Calculated Molecular Weight: 123 kDa
• Observed Molecular Weight: 100-120 kDa, 140-160 kDa
• GenBank Accession Number: BC015186
• Gene Symbol: PDGFR alpha
• Gene ID (NCBI): 5156
• RRID: AB_2162343
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival and chemotaxis. Mutations in PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Publications

Species	Application	Title
human	WB,IF	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition; Authors - Tiffany L. Dill
mouse	IF	Development; The long noncoding RNA Meg3 regulates myoblast plasticity and muscle regeneration through epithelial-mesenchymal transition.; Authors - Tiffany L Dill
mouse	WB	Metabolism; The vitamin D receptor regulates miR-140-5p and targets the MAPK pathway in bone development.; Authors - Wenting Luo

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Tom (Verified Customer) (11-25-2021)

HEK293 lysate - some non-specific bands below real band observed.

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293

PDGFR alpha Antibody Western Blot validation (1/1000 dilution) in HEK293 (Cat no:60045-1-Ig)

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