PDGFR alpha Polyclonal antibody

PDGFR alpha Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 10945-1-AP

Synonyms

CD140A, CD140a antigen, PDGF R alpha, PDGF receptor alpha, PDGFR2, PDGFRA, PDGFRα, PDGFR-α, Rhe PDGFRA

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Validation Data Gallery

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  IHC staining of mouse liver using 10945-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse liver using 10945-1-AP (PDGFRA antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive IHC detected in: mouse liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

10945-1-AP targets PDGFR alpha in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDGFR alpha fusion protein Ag1385
• Full Name: platelet-derived growth factor receptor, alpha polypeptide
• Calculated Molecular Weight: 123 kDa
• GenBank Accession Number: BC015186
• Gene Symbol: PDGFR alpha
• Gene ID (NCBI): 5156
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet-derived growth factor receptor alpha (PDGFRA, also known as CD140a) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. PDGFRA is implicated in the regulation of embryonic development, tumorigenesis, cell proliferation, survival, and chemotaxis. Mutations in the PDGFRA gene have been associated with idiopathic hypereosinophilic syndrome, somatic and familial gastrointestinal stromal tumors, and a variety of other cancers.

Publications

Species	Application	Title
human	WB	Oncol Res; GIPC1 promotes tumor growth and migration in gastric cancer via activating PDGFR/PI3K/AKT signaling; Authors - Tingting Li