PDGF-BB Recombinant antibody, PBS Only

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  WB analysis using 83214-5-RR (same clone as 83214-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83214-5-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 83214-5-RR (same clone as 83214-5-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83214-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83214-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83214-5-RR against Human PDGFB were performed. The affinity constant is 16.9 nM.

PDGF-BB Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 83214-5-PBS

Clone No.230486A7

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PDGFB, PDGFbb, PDGF2, PDGF subunit B, PDGF 2

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Product Information

83214-5-PBS targets PDGF-BB in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDGF-BB fusion protein Ag25986
• Full Name: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
• Calculated Molecular Weight: 241 aa, 27 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC029822
• Gene Symbol: PDGFB
• Gene ID (NCBI): 5155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P01127-1
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Platelet-derived growth factor (PDGF) belongs to the family of growth factors consisting of four secreted extracellular ligands including PDGF-A, -B, -C and -D. These isoforms, PDGF-AA, PDGF-AB, PDGF-BB, PDGF-CC and PDGF-DD, act via two receptor tyrosine kinases, PDGF receptors alpha and beta. PDGF isoforms stimulate growth, survival and motility of mesenchymal cells and certain other cell type. They have important functions during embryonal development and in the control of tissue homeostasis in the adult. PDGF-BB is one of the most recently studied isoforms and it plays an important role in skeletal development. PDGF-BB stimulates cell-mediated collagen gel contraction. PDGF-BB modulates endothelial proliferation and angiogenesis in vitro via PDGF beta-receptors.