PDGFB Recombinant antibody, PBS Only

PDGFB Uni-rAb^TM Recombinant Antibody for ELISA, WB

Host / Isotype

Rabbit / IgG

Reactivity

rat, mouse, human

Applications

ELISA, WB

Conjugate

Unconjugated

CloneNo.

230486A7

Cat no : 83214-5-PBS

Synonyms

PDGFbb, PDGF2, PDGF subunit B, PDGF 2, Becaplermin

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Validation Data Gallery

• 

  WB analysis using 83214-5-RR (same clone as 83214-5-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83214-5-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 83214-5-RR (same clone as 83214-5-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 83214-5-RR (PDGFB antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83214-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83214-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83214-5-RR against Human PDGFB were performed. The affinity constant is below 1 pM.

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Product Information

The immunogen of 83214-5-PBS is PDGFB Fusion Protein expressed in E. coli.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDGFB fusion protein Ag25986
• Full Name: platelet-derived growth factor beta polypeptide (simian sarcoma viral (v-sis) oncogene homolog)
• Calculated Molecular Weight: 241 aa, 27 kDa
• GenBank Accession Number: BC029822
• Gene Symbol: PDGFB
• Gene ID (NCBI): 5155
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.