PDGFA Polyclonal antibody

PDGFA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 26940-1-AP

Synonyms

PDGF-1, PDGF1, PDGF subunit A, PDGF AA, PDGF A

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Validation Data Gallery

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  WB analysis using 26940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 26940-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26940-1-AP (PDGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, mouse kidney tissue, rat kidney tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26940-1-AP targets PDGFA in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDGFA fusion protein Ag25643
• Full Name: platelet-derived growth factor alpha polypeptide
• Calculated Molecular Weight: 196 aa, 22 kDa
• Observed Molecular Weight: 13-16 kDa
• GenBank Accession Number: BC146594
• Gene Symbol: PDGFA
• Gene ID (NCBI): 5154
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDGFA is a member of the platelet-derived growth factor family. Four members of this family are mitogenic factors for cells of mesenchymal origin, and they are characterized by a motif of eight cysteines. PDGFA plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, and chemotaxis. It is also required for normal lung alveolar septum formation during embryogenesis, development of the gastrointestinal tract, and spermatogenesis. PDGFA is also involved in oligodendrocyte development and the myelination process in the spinal cord and cerebellum. PDGF-A protein monomer is about 13-16 kDa, and PDGF-AA is a 28.5 kDa A chain homodimer.