PDE7B Polyclonal antibody

• Featured Product
• KD/KO Validated

PDE7B Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 17771-1-AP

Synonyms

phosphodiesterase 7B, EC:3.1.4.53, cAMP-specific phosphodiesterase 7B, 3',5'-cyclic-AMP phosphodiesterase 7B

---

Validation Data Gallery

• 

  WB analysis of HeLa using 17771-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 17771-1-AP (PDE7B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17771-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17771-1-AP (PDE7B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human brain using 17771-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17771-1-AP (PDE7B antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17771-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17771-1-AP (PDE7B antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 17771-1-AP

  Immunofluorescent analysis of HepG2 cells, using PDE7B antibody 17771-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: human brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Product Information

17771-1-AP targets PDE7B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE7B fusion protein Ag12184
• Full Name: phosphodiesterase 7B
• Calculated Molecular Weight: 450 aa, 52 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC075082
• Gene Symbol: PDE7B
• Gene ID (NCBI): 27115
• RRID: AB_2268032
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE7B(cAMP-specific 3',5'-cyclic phosphodiesterase 7B) belongs to the cyclic nucleotide phosphodiesterase family and PDE7 subfamily,which regulate intracellular levels of cAMP and cGMP, cyclic nucleotides that play important roles as second messengers in multiple physiologic processes. PDE7B gene is expressed predominantly in the brain and in a number of other tissues including liver, heart, thyroid glands, and skeletal muscle, but not expressed in blood leukocytes (PMID:12388353).

Publications

Species	Application	Title
human	WB, IF	Neurobiol Aging; Silencing phosphodiesterase 7B gene by lentiviral-shRNA interference attenuates neurodegeneration and motor deficits in hemiparkinsonian mice.; Authors - Jose A Morales-Garcia ; KD Validated
rat	WB	Stem Cells; Phosphodiesterase7 Inhibition Activates Adult Neurogenesis in Hippocampus and Subventricular Zone in vitro and in vivo.; Authors - Jose A Morales-Garcia
rat	WB	Stem Cells Transl Med; Phosphodiesterase 7 inhibition induces dopaminergic neurogenesis in hemiparkinsonian rats.; Authors - Jose A Morales-Garcia
human	WB,IHC,IF	BMC Cancer; Methylation-regulated tumor suppressor gene PDE7B promotes HCC invasion and metastasis through the PI3K/AKT signaling pathway; Authors - Yuanxiao Du ; KO Validated