PDE6D Polyclonal antibody

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• KD/KO Validated

PDE6D Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 28573-1-AP

Synonyms

PDED

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Validation Data Gallery

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  WB analysis using 28573-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28573-1-AP (PDE6D antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 28573-1-AP

  WB result of PDE6D antibody (28573-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDE6D transfected HeLa cells.

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, human placenta tissue, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

28573-1-AP targets PDE6D in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE6D fusion protein Ag29563
• Full Name: phosphodiesterase 6D, cGMP-specific, rod, delta
• Calculated Molecular Weight: 17 KDa
• Observed Molecular Weight: 14-17 kDa
• GenBank Accession Number: NM_002601
• Gene Symbol: PDE6D
• Gene ID (NCBI): 5147
• RRID: AB_2881173
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE6D, also known as PDED, belongs to the PDE6D/unc-119 family. PDE6D is a ubiquitously expressed prenyl-binding protein. By binding to prenyl moieties, PDE6D mediates the shuttling of prenylated proteins between membranes. Thereby, it regulates the membrane association of Ras and Rap GTPases (PMID: 36672247). In addition, research has shown that PDE6D is overexpressed in liver cancer and correlated with tumor stages, grading, and ERK activation (PMID: 30901922). The calculated molecular weight of PDE6D is 17 kDa.