PDE6B Polyclonal antibody

PDE6B Polyclonal Antibody for WB, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 22063-1-AP

Synonyms

CSNB3, GMP PDE beta, PDE6B, PDEB, rd1, RP40

---

Validation Data Gallery

• 

  WB analysis of mouse eye tissue using 22063-1-AP

  WB results of PDE6B antibody (22063-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.

• 

  WB analysis of mouse eye using 22063-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 22063-1-AP (PDE6B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat eye using 22063-1-AP

  rat eye tissue were subjected to SDS PAGE followed by western blot with 22063-1-AP (PDE6B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 22063-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 22063-1-AP (PDE6B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Y79 using 22063-1-AP

  IP result of anti-PDE6B (IP:22063-1-AP, 4ug; Detection:22063-1-AP 1:1000) with Y79 cells lysate 2000ug.

• 

  IF Staining of mouse eye using 22063-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse eye tissue using PDE6B antibody (22063-1-AP) at dilution of 1:100 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: mouse eye tissue, A549 cells, rat eye tissue
• Positive IP detected in: Y79 cells
• Positive IF-P detected in: mouse eye tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Product Information

The immunogen of 22063-1-AP is PDE6B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE6B fusion protein Ag17139
• Full Name: phosphodiesterase 6B, cGMP-specific, rod, beta
• Calculated Molecular Weight: 854 aa, 98 kDa
• Observed Molecular Weight: 98 kDa
• GenBank Accession Number: BC000249
• Gene Symbol: PDE6B
• Gene ID (NCBI): 5158
• RRID: AB_2619668
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE6 is a cGMP-specific PDE family and presents multicomponent enzyme complexes. The rod PDE6 enzyme is comprised of two catalytic subunits (PDE6A and PDE6B) and it is expressed in human lungs (PMID:20979602). PDE6B(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit beta) is also named as PDEB and belongs to the cyclic nucleotide phosphodiesterase family. PDE6B also has a C-terminal CAAX motif for posttranslational processing involving lipidation, proteolysis, and carboxymethylation (PMID:8394243). Defects in PDE6B are the cause of retinitis pigmentosa type 40 (RP40)(PMID:22334370) and congenital stationary night blindness autosomal dominant type 2 (CSNBAD2) (PMID:8075643).

Publications

Species	Application	Title
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	WB,IF	FEBS J; Deletion of Emc1 in photoreceptor cells causes retinal degeneration in mice; Authors - Xiao Li
zebrafish	WB	J Biol Chem; Pathogenic Mutations in Retinitis Pigmentosa 2 Predominantly Result in Loss of RP2 Protein Stability in Human and Zebrafish.; Authors - Fei Liu
mouse	WB,IF	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta
mouse	WB	Dev Biol; Luteinizing hormone signaling phosphorylates and activates the cyclic GMP phosphodiesterase PDE5 in mouse ovarian follicles, contributing an additional component to the hormonally induced decrease in cyclic GMP that reinitiates meiosis.; Authors - Jeremy R Egbert
mouse	WB, IHC	PLoS One; Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance.; Authors - Poppy Datta