PDE6A Monoclonal antibody

PDE6A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B3B4

Cat no : 67832-1-Ig

Synonyms

CGPR A, GMP PDE alpha, PDE V B1, PDE6 alpha, PDE6A, PDEA

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Validation Data Gallery

• 

  WB analysis of mouse eye using 67832-1-Ig

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse retina using 67832-1-Ig

  mouse retina tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat eye using 67832-1-Ig

  rat eye tissue were subjected to SDS PAGE followed by western blot with 67832-1-Ig (PDE6A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse eye tissue, rat eye tissue, mouse retina tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67832-1-Ig targets PDE6A in WB, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

• Tested Reactivity: Human, Mouse, Rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDE6A fusion protein Ag16741
• Full Name: phosphodiesterase 6A, cGMP-specific, rod, alpha
• Calculated Molecular Weight: 860 aa, 100 kDa
• Observed Molecular Weight: 100 kDa
• GenBank Accession Number: BC035909
• Gene Symbol: PDE6A
• Gene ID (NCBI): 5145
• RRID: AB_2918594
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE6A(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha) is also named as GMP-PDE alpha, PDE V-B1, PDEA and belongs to the cyclic nucleotide phosphodiesterase family. PDE6A contains an open reading frame capable of coding for a polypeptide of 859 amino acids and about 100 kDa. It is a subunit of a key phototransduction enzyme which participates in processes of transmission and amplification of the visual signal. The expression of PDE6 alpha in retina is essential for normal expression of PDE6 beta and PDE6 gama.