PDE6A Polyclonal antibody

PDE6A Polyclonal Antibody for WB, IHC, IF, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 21200-1-AP

Synonyms

PDEA, PDE V-B1, PDE V B1, GMP-PDE alpha, EC:3.1.4.35

---

Validation Data Gallery

• 

  WB analysis using 21200-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21200-1-AP (PDE6A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye tissue using 21200-1-AP

  WB results of PDE6A antibody (21200-1-AP) with WT mouse Eye and Prph2 (Rds) mutant mouse Eye (Negative control). Courtesy of Seongjin Seo, PhD, University of Iowa College of Medicine.

• 

  WB analysis of human brain using 21200-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 21200-1-AP (PDE6A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse eye using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21200-1-AP (PDE6A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21200-1-AP (PDE6A antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovarian cancer slide using 21200-1-AP (PDE6A antibody) at dilution of 1:4000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse eye using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 21200-1-AP (PDE6A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human colon cancer using 21200-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 21200-1-AP (PDE6A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of retinal tissue using 21200-1-AP

  IF stain of PDE6a antibody with retinal tissue by a customer. (PDE6a is in red and nuclei are blue).

---

Tested Applications

• Positive WB detected in: mouse eye tissue, human brain tissue, rat eye tissue
• Positive IHC detected in: mouse eye tissue, human colon cancer tissue, human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: retinal tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF): IF : 1:50-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

21200-1-AP targets PDE6A in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE6A fusion protein Ag15562
• Full Name: phosphodiesterase 6A, cGMP-specific, rod, alpha
• Calculated Molecular Weight: 860 aa, 100 kDa
• Observed Molecular Weight: 100-110 kDa
• GenBank Accession Number: BC035909
• Gene Symbol: PDE6A
• Gene ID (NCBI): 5145
• RRID: AB_10733345
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE6A(Rod cGMP-specific 3',5'-cyclic phosphodiesterase subunit alpha) is also named as GMP-PDE alpha, PDE V-B1, PDEA and belongs to the cyclic nucleotide phosphodiesterase family. PDE6A contains an open reading frame capable of coding for a polypeptide of 859 amino acids and about 100 kDa. It is a subunit of a key phototransduction enzyme which participates in processes of transmission and amplification of the visual signal. The expression of PDE6 alpha in retina is essential for normal expression of PDE6 beta and PDE6 gama.

Publications

Species	Application	Title
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
mouse	WB	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta
rat	WB	Toxicol Appl Pharmacol; Ocular toxicity of AUY922 in pigmented and albino rats.; Authors - Danielle Roman
mouse	WB	Dev Biol; Luteinizing hormone signaling phosphorylates and activates the cyclic GMP phosphodiesterase PDE5 in mouse ovarian follicles, contributing an additional component to the hormonally induced decrease in cyclic GMP that reinitiates meiosis.; Authors - Jeremy R Egbert
mouse	WB	PLoS One; Mouse models of human ocular disease for translational research.; Authors - Mark P Krebs
	IHC,WB	Exp Eye Res; Characterization of lncRNA and mRNA profiles in ciliary body in experimental myopia; Authors - Shanshan Wu