PDE4D Monoclonal antibody, PBS Only

PDE4D Monoclonal Antibody for WB, IF, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1C4B9

Cat no : 67062-1-PBS

Synonyms

DPDE3, HSPDE4D, PDE43, PDE4D, PDE4DN2, STRK1

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Validation Data Gallery

• 

  WB analysis using 67062-1-Ig (same clone as 67062-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67062-1-Ig (same clone as 67062-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of pig cerebellum using 67062-1-Ig (same clone as 67062-1-PBS)

  pig cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67062-1-Ig (same clone as 67062-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat cerebellum using 67062-1-Ig (same clone as 67062-1-PBS)

  rat cerebellum tissue were subjected to SDS PAGE followed by western blot with 67062-1-Ig (PDE4D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 67062-1-Ig (same clone as 67062-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PDE4D antibody (67062-1-Ig, Clone: 1C4B9 ) at dilution of 1:1600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67062-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67062-1-PBS targets PDE4D in WB, IF, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PDE4D fusion protein Ag8302
• Full Name: phosphodiesterase 4D, cAMP-specific (phosphodiesterase E3 dunce homolog, Drosophila)
• Calculated Molecular Weight: 809 aa, 91 kDa
• Observed Molecular Weight: 91-120 kDa, 66-84 kDa
• GenBank Accession Number: BC036319
• Gene Symbol: PDE4D
• Gene ID (NCBI): 5144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.