PDE4B Polyclonal antibody

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  WB analysis using 31730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 31730-1-AP (PDE4B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

PDE4B Polyclonal Antibody for WB, ELISA

Cat No. 31730-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PDEIVB, PDE4B5, PDE32, DPDE4

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

31730-1-AP targets PDE4B in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE4B fusion protein Ag35552
• Full Name: phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila)
• Calculated Molecular Weight: 736 aa, 83 kDa
• Observed Molecular Weight: 83 kDa
• GenBank Accession Number: BC105040
• Gene Symbol: PDE4B
• Gene ID (NCBI): 5142
• RRID: AB_3670101
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: Q07343
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PDE4B, also known as DPDE4, is a member of the PDE family of type IV cAMP-specific cyclic nucleotides. PDE4B regulates the cellular concentration of cyclic nucleotides and thus plays a role in signal transduction of inflammatory factors. Among all subtypes of PDE4, PDE4B is closely associated with cancer and has a major contribution to the role in hematological malignancies. PDE4B is distributed in multiple tissues, especially myeloid cells (PMID: 35899614, PMID: 36278172).