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PDE4A Polyclonal antibody

PDE4A Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16226-1-AP

Synonyms

DPDE2, PDE4, PDE46, PDE4A



Tested Applications

Positive WB detected inhuman brain tissue, HeLa cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16226-1-AP targets PDE4A in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDE4A fusion protein Ag8018
Full Name phosphodiesterase 4A, cAMP-specific (phosphodiesterase E2 dunce homolog, Drosophila)
Calculated Molecular Weight 98 kDa
Observed Molecular Weight 125-130 kDa
GenBank Accession NumberBC019864
Gene Symbol PDE4A
Gene ID (NCBI) 5141
RRIDAB_2252090
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDE4A antibody 16226-1-APDownload protocol
IHC protocol for PDE4A antibody 16226-1-APDownload protocol
IP protocol for PDE4A antibody 16226-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Br J Pharmacol

Involvement of 3',5'-cyclic inosine monophosphate in cystathionine γ-lyase-dependent regulation of the vascular tone.

Authors - Emma Mitidieri
mouseWB

Int J Mol Sci

Phosphodiesterases Expression during Murine Cardiac Development.

Authors - Thays Maria da Conceição Silva Carvalho
mouseWB

Biochem Pharmacol

DC591017, a phosphodiesterase-4 (PDE4) inhibitor with robust anti-inflammation through regulating PKA-CREB signaling.

Authors - Heng Li
humanWB,IHC

Cell Signal

Phosphodiesterase 4 in inflammatory diseases: Effects of apremilast in psoriatic blood and in dermal myofibroblasts through the PDE4/CD271 complex.

Authors - Peter H Schafer
mouseWB

Cell Signal

RGS10 physically and functionally interacts with STIM2 and requires store-operated calcium entry to regulate proinflammatory gene expression in microglia.

Authors - Menbere Wendimu
humanIHC

Drugs R D

Apremilast Normalizes Gene Expression of Inflammatory Mediators in Human Keratinocytes and Reduces Antigen-Induced Atopic Dermatitis in Mice.

Authors - Peter H Schafer