PDE3B Polyclonal antibody

PDE3B Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 25290-1-AP

Synonyms

EC:3.1.4.17, Cyclic GMP-inhibited phosphodiesterase B, cGMP-inhibited 3',5'-cyclic phosphodiesterase 3B, cGIPDE1, CGI-PDE B

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Validation Data Gallery

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  WB analysis of HL-60 using 25290-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25290-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25290-1-AP (PDE3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 25290-1-AP

  IP result of anti-PDE3B (IP:25290-1-AP, 5ug; Detection:25290-1-AP 1:300) with HeLa cells lysate 800ug.

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Tested Applications

• Positive WB detected in: HL-60 cells, HeLa cells
• Positive IP detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

25290-1-AP targets PDE3B in WB, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE3B fusion protein Ag18064
• Full Name: phosphodiesterase 3B, cGMP-inhibited
• Calculated Molecular Weight: 1112 aa, 124 kDa
• Observed Molecular Weight: 124 kDa
• GenBank Accession Number: BC136565
• Gene Symbol: PDE3B
• Gene ID (NCBI): 5140
• RRID: AB_2880010
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB	Int J Mol Sci; Exogenous H2S Attenuates Hypertension by Regulating Renin Exocytosis under Hyperglycaemic and Hyperlipidaemic Conditions; Authors - Ning Liu