PDE12 Polyclonal antibody, PBS Only

PDE12 Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12753-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human brain using 12753-1-AP (same clone as 12753-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.

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  WB analysis using 12753-1-AP (same clone as 12753-1-PBS)

  THP-1 cells were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 12753-1-AP (same clone as 12753-1-PBS)

  IP result of anti-PDE12 (IP:12753-1-AP, 4ug; Detection:12753-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12753-1-AP (same clone as 12753-1-PBS)

  Immunofluorescent analysis of HepG2 cells, using PDE12 antibody 12753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.

• 

  FC experiment of HepG2 using 12753-1-AP (same clone as 12753-1-PBS)

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug PDE12 Polyclonal antibody (12753-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.

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Product Information

12753-1-PBS targets PDE12 in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDE12 fusion protein Ag3217
• Full Name: phosphodiesterase 12
• Calculated Molecular Weight: 472 aa, 52 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC034978
• Gene Symbol: PDE12
• Gene ID (NCBI): 201626
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.