WB analysis of human brain using 12753-1-AP (same clone as 12753-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
human brain tissue were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
WB analysis using 12753-1-AP (same clone as 12753-1-PBS)
THP-1 cells were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
THP-1 cells were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
IP experiment of mouse brain using 12753-1-AP (same clone as 12753-1-PBS)
IP result of anti-PDE12 (IP:12753-1-AP, 4ug; Detection:12753-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
IP result of anti-PDE12 (IP:12753-1-AP, 4ug; Detection:12753-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
IF Staining of HepG2 using 12753-1-AP (same clone as 12753-1-PBS)
Immunofluorescent analysis of HepG2 cells, using PDE12 antibody 12753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
Immunofluorescent analysis of HepG2 cells, using PDE12 antibody 12753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 12753-1-AP (same clone as 12753-1-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug PDE12 Polyclonal antibody (12753-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug PDE12 Polyclonal antibody (12753-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
WB analysis of human brain using 12753-1-AP (same clone as 12753-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
WB analysis using 12753-1-AP (same clone as 12753-1-PBS)
THP-1 cells were subjected to SDS PAGE followed by western blot with 12753-1-AP (PDE12 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
IP Figures
IP experiment of mouse brain using 12753-1-AP (same clone as 12753-1-PBS)
IP result of anti-PDE12 (IP:12753-1-AP, 4ug; Detection:12753-1-AP 1:500) with mouse brain tissue lysate 4000ug. This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
IF/ICC Figures
IF Staining of HepG2 using 12753-1-AP (same clone as 12753-1-PBS)
Immunofluorescent analysis of HepG2 cells, using PDE12 antibody 12753-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
FC (INTRA) Figures
FC experiment of HepG2 using 12753-1-AP (same clone as 12753-1-PBS)
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug PDE12 Polyclonal antibody (12753-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12753-1-PBS in a different storage buffer formulation.
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
PDE12 Polyclonal antibody
Catalog Number
12753-1-PBS
Citations
-
Dilutions
Applications
WB, IF/ICC, FC (Intra), IP, Indirect ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
