PDCL Polyclonal antibody

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  WB analysis using 16057-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16057-1-AP (PDCL antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCI-H1299 using 16057-1-AP

  NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 16057-1-AP (PDCL antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 16057-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16057-1-AP (PDCL antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human placenta using 16057-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 16057-1-AP (PDCL antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 16057-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16057-1-AP (PDCL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PDCL Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16057-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

PDCL, PhLP, phosducin like, Phosducin like protein

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Tested Applications

• Positive WB detected in: HEK-293 cells, NCI-H1299 cells, Jurkat cells
• Positive IHC detected in: human placenta tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16057-1-AP targets PDCL in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDCL fusion protein Ag9029
• Full Name: phosducin-like
• Calculated Molecular Weight: 301 aa, 34 kDa
• Observed Molecular Weight: 40-42 kDa
• GenBank Accession Number: BC017227
• Gene Symbol: PDCL
• Gene ID (NCBI): 5082
• RRID: AB_2236943
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q13371
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
rat	WB	Acta Physiol (Oxf); ATP6V1A is required for synaptic rearrangements and plasticity in murine hippocampal neurons; Authors - Alessandro Esposito