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  • KD/KO Validated

Alix Polyclonal antibody

Alix Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (1)





Cat no : 12422-1-AP


AIP1, ALG 2 interacting protein 1, Alix, DRIP4, HP95, KIAA1375, PDCD6 interacting protein, PDCD6IP

Tested Applications

Positive WB detected inHEK-293 cells, human urine exosomes tissue, Jurkat cells, mouse liver tissue, rat liver tissue, NIH/3T3 cells, HeLa cells
Positive IP detected inJurkat cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12422-1-AP targets Alix in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, monkey
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Alix fusion protein Ag3074
Full Name programmed cell death 6 interacting protein
Calculated Molecular Weight 868 aa, 96 kDa
Observed Molecular Weight 96 kDa, 75-80 kDa
GenBank Accession NumberBC020066
Gene Symbol PDCD6IP
Gene ID (NCBI) 10015
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ALG-2-interacting protein 1 (ALIX), also known as AIP1 or Hp95, is encoded by PDCD6IP gene and is involved in cell death through mechanisms involving its binding partner ALG-2 (apoptosis-linked gene-2). ALG-2 is a 22-kDa protein containing five serially repetitive EF-hand structures and is defined as a regulator of calcium-induced apoptosis following endoplasmic reticulum (ER) stress. ALIX interacts with ALG-2 through its C-terminal proline-rich region and participates in formation of multivesicular bodies. Recent finding suggest that ALIX is a critical component of caspase 9 activation and apoptosis triggered by calcium. The alix antibody recognizes an additional band of 75-80 kDa which has also been observed in cells and exosomes.


Product Specific Protocols
WB protocol for Alix antibody 12422-1-APDownload protocol
IHC protocol for Alix antibody 12422-1-APDownload protocol
IF protocol for Alix antibody 12422-1-APDownload protocol
IP protocol for Alix antibody 12422-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Kamal (Verified Customer) (02-15-2024)

Strong bands appeared between 70 and 100 kDa.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10000
  • Cell Tissue Type: Mouse liver tissue

Guorong (Verified Customer) (03-22-2022)

Excellent performance with a specific band of expected size

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: ARPE-19
Alix Antibody Western Blot validation (1:5000 dilution) in ARPE-19 (Cat no:12422-1-AP)

Susmita (Verified Customer) (02-11-2022)

all antibodies from Proteintech works great for me

  • Applications: Western Blot, Immunoprecipitation
  • Primary Antibody Dilution: WB: 1: 2000

Jorge (Verified Customer) (05-15-2019)

Gave a very strong signal using the lowest recommended antibody dilution for Western Blot, diluted it to 1:2000 and worked just as well with a low protein detection substrate. Detected exosomes in fraction 7 of a sucrose gradient as expected.

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000 in 5% BSA-TBST
  • Cell Tissue Type: Mouse 4T1 cells
Alix Antibody Western Blot, validation (1:1000 in 5% BSA-TBST dilution) in Mouse 4T1 cells (Cat no:12422-1-AP)