PDCD4 Recombinant antibody

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  WB analysis using 84162-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84162-3-RR (PDCD4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of HeLa using 84162-3-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PDCD4 Recombinant antibody (84162-3-RR, Clone:241463E3) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84162-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84162-3-RR against Human PDCD4 were performed. The affinity constant is 1.54 nM.

PDCD4 Uni-rAb^TM Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84162-3-RR

Clone No.241463E3

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

Tis, PDCD 4, Nuclear antigen H731-like, H731, Dug

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HeLa cells, HUVEC cells, MCF-7 cells, HEK-293 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84162-3-RR targets PDCD4 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PDCD4 fusion protein Ag3288
• Full Name: programmed cell death 4 (neoplastic transformation inhibitor)
• Calculated Molecular Weight: 469 aa, 52 kDa
• Observed Molecular Weight: 54-64 kDa
• GenBank Accession Number: BC026104
• Gene Symbol: PDCD4
• Gene ID (NCBI): 27250
• RRID: AB_3671724
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q53EL6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.