PDCD4 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PDCD4 Polyclonal Antibody for WB, IHC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12587-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis using 12587-1-AP (same clone as 12587-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK293 cells using 12587-1-AP (same clone as 12587-1-PBS)

  WB result of PDCD4 antibody (12587-1-AP, 1:4000) with si-control and si-PDCD4 transfected HEK293 cells. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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  WB analysis of MCF-7 using 12587-1-AP (same clone as 12587-1-PBS)

  MCF7 cells were subjected to SDS PAGE followed by western blot with 12587-1-AP (PDCD4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12587-1-AP (same clone as 12587-1-PBS)

  IP result of anti-PDCD4 (IP:12587-1-AP, 4ug; Detection:12587-1-AP 1:3000) with HeLa cells lysate 4000ug. This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 12587-1-AP (same clone as 12587-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12587-1-AP (PDCD4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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  FC experiment of MCF-7 using 12587-1-AP (same clone as 12587-1-PBS)

  1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human PDCD4 (12587-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 12587-1-PBS in a different storage buffer formulation.

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Product Information

12587-1-PBS targets PDCD4 in WB, IHC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PDCD4 fusion protein Ag3288
• Full Name: programmed cell death 4 (neoplastic transformation inhibitor)
• Calculated Molecular Weight: 469 aa, 52 kDa
• Observed Molecular Weight: 54-64 kDa
• GenBank Accession Number: BC026104
• Gene Symbol: PDCD4
• Gene ID (NCBI): 27250
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Programmed cell death 4 (Pdcd4) is a novel tumor suppressor that inhibits translation, progression and invasion. It was first identified as being differnetially upregulated during apoptosis. Pdcd4 interferes with the activity of the eukaryotic initiation factor (eIF) 4A by displacing the scaffold protein eIF4G from its binding to the RNA helicase eIF4A. The calculated molecular wight of unmodified PDCD4 protein is 52 kDa, but the modified protein is about 54-64 kDa.