PDAP1 Polyclonal antibody

PDAP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 15081-1-AP

Synonyms

PDGF-associated protein, PDGFA-associated protein 1, PDGFA associated protein 1, PDGF associated protein, HASPP28



Tested Applications

Positive WB detected inMCF7 cells, MCF-7 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

15081-1-AP targets PDAP1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PDAP1 fusion protein Ag7155
Full Name PDGFA associated protein 1
Calculated Molecular Weight 21 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC000684
Gene Symbol PDAP1
Gene ID (NCBI) 11333
RRIDAB_2159306
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PDAP1 antibody 15081-1-APDownload protocol
IHC protocol for PDAP1 antibody 15081-1-APDownload protocol
IF protocol for PDAP1 antibody 15081-1-APDownload protocol
IP protocol for PDAP1 antibody 15081-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Proteomics Clin Appl

SILAC-based quantitative proteomic analysis of gastric cancer secretome.

Authors - Marimuthu Arivusudar A
humanWB

Cancer Sci

Pancreatic ductal adenocarcinoma cells regulated the gemcitabine-resistance function of CAFs by LINC00460

Authors - Xiao‐Xu Zhu