PD-L1/CD274 Recombinant antibody, PBS Only

PD-L1/CD274 Uni-rAb^TM Recombinant Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3G4

Cat no : 82719-13-PBS

Synonyms

CD274, PD-L1, PD L1, hPD-L1, B7-H1

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Validation Data Gallery

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  IHC staining of human tonsillitis using 82719-13-RR (same clone as 82719-13-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82719-13-RR (PD-L1/CD274 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82719-13-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 82719-13-RR (same clone as 82719-13-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82719-13-RR (PD-L1/CD274 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82719-13-PBS in a different storage buffer formulation.

• 

  IHC staining of human tonsillitis using 82719-13-RR (same clone as 82719-13-PBS)

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 82719-13-RR (PD-L1/CD274 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82719-13-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 82719-13-RR

  Biolayer interferometry (BLl) kinetic assays of 82719-13-RR against Human PD-L1/CD274 were performed. The affinity constant is below 1 pM.

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Product Information

82719-13-PBS targets PD-L1/CD274 in IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PD-L1/CD274 fusion protein Ag12432
• Full Name: CD274 molecule
• Calculated Molecular Weight: 290 aa, 33 kDa
• GenBank Accession Number: BC074984
• Gene Symbol: PD-L1
• Gene ID (NCBI): 29126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Programmed cell death ligand 1 (PD-L1, CD274, or B7-H1), is the first member of B7 family to be discovered. B7 family molecules are type I transmembrane proteins belonging to the immunoglobulin superfamily. In concert with their CD28 family receptors, the B7s are key regulators of the adaptive immune response. PD-L1 is suggested as a negative regulator of T and B cell, and plays important role in mediating tolerance of lymphocytes to self-antigens. It is also involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PD-1-independent manner. PD-L1 is a 290 aa transmembrane protein with a calculated molecular weight of 33 kDa, it is predicted to be 27-30 kDa after signal peptide cleavage (PMID: 25609200; 17076679). The apparent molecular weight has also been reported as 45-70 kDa, major glycosylated form of 45-50 kDa and multiple post-translational modifications form of 65-70 kDa (PMID: 18760278; 16493058).