Anti-Human PD-1/CD279 Rabbit Recombinant Antibody

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  IHC staining of human tonsillitis using 98068-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98068-1-RR (PD-1/CD279 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 98068-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 98068-1-RR (PD-1/CD279 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of human PBMCs using 98068-1-RR

  1x10^6 unstimulated or PHA stimulated human PBMCs were surface stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD3. Cells were not fixed.

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  FC experiment of human PBMCs using 98068-1-RR

  1x10^6 PHA stimulated human PBMCs were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11)(left) or 0.25 ug PE Anti-Human PD-1/CD279 (J110) (PE-65119, Clone: J110)(right) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), Cells were then stained with APC Anti-Human CD3 (OKT3) Mouse IgG2a Recombinant Antibody (APC-65569, Clone: OKT3). Cells were not fixed.

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  FC experiment of human PBMCs using 98068-1-RR

  1x10^6 unstimulated (black) or PHA stimulated human PBMCs (red) were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue). Cells were not fixed.

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  FC experiment of human PBMCs using 98068-1-RR

  1x10^6 unstimulated (black) or PHA treated human PBMCs were stained with 0.25 ug Anti-Human PD-1/CD279 Rabbit Recombinant Antibody (98068-1-RR, Clone:240724G11)(red) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) or 0.25 ug PE Anti-Human PD-1/CD279 (J110) (PE-65119, Clone: J110)(green). 1x10^6 PHA treated human PBMCs were stained with Isotype Control(blue) . Cells were not fixed.

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  Affinity and Kinetic Characterization of 98068-1-RR

  Biolayer interferometry (BLl) kinetic assays of 98068-1-RR against Human PD-1/CD279 were performed. The affinity constant is 0.966 nM.

PD-1/CD279 Uni-rAb^TM Recombinant Antibody for IHC, FC

Cat No. 98068-1-RR

Clone No.240724G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, FC

Synonyms

CD279, PD1, PD-1, 240724G11, PD 1

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Tested Applications

• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: human PBMCs

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:500-1:2000

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98068-1-RR targets PD-1/CD279 in IHC, FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: programmed cell death 1
• Calculated Molecular Weight: 288 aa, 32 kDa
• GenBank Accession Number: BC074740
• Gene Symbol: PD-1
• Gene ID (NCBI): 5133
• RRID: AB_3672215
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q15116
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

Programmed cell death 1 (PD-1, also known as CD279) is an immunoinhibitory receptor that belongs to the CD28/CTLA-4 subfamily of the Ig superfamily. It is a 288 amino acid (aa) type I transmembrane protein composed of one Ig superfamily domain, a stalk, a transmembrane domain, and an intracellular domain containing an immunoreceptor tyrosine-based inhibitory motif (ITIM) as well as an immunoreceptor tyrosine-based switch motif (ITSM) (PMID: 18173375). PD-1 is expressed during thymic development and is induced in a variety of hematopoietic cells in the periphery by antigen receptor signaling and cytokines (PMID: 20636820). Engagement of PD-1 by its ligands PD-L1 or PD-L2 transduces a signal that inhibits T-cell proliferation, cytokine production, and cytolytic function (PMID: 19426218). It is critical for the regulation of T cell function during immunity and tolerance. Blockade of PD-1 can overcome immune resistance and also has been shown to have antitumor activity (PMID: 22658127; 23169436). It has been reported that PD-1 is heavily glycosylated and migrates with an apparent molecular mass of 47-55 kDa on SDS-PAGE , which is larger than its predicted mass of 32 kDa (PMID: 8671665; 17640856; 17003438).