PD-1/CD279 Recombinant antibody, PBS Only (Detector)

PD-1/CD279 Uni-rAb^TM Recombinant Antibody for Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Cytometric bead array, Sandwich ELISA, Indirect ELISA, Sample test

Conjugate

Unconjugated

CloneNo.

240724G9

Cat no : 83632-4-PBS

Synonyms

CD279, PD1, PD-1, 240724G9, PD 1

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Validation Data Gallery

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  Sandwich ELISA standard curve of MP00612-4

  Sandwich ELISA standard curve of MP00612-4, Human PD-1/CD279 Recombinant Matched Antibody Pair - PBS only. 83632-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Eg0974. 83632-4-PBS was HRP conjugated as the detection antibody. Range: 31.25-2000 pg/mL

• 

  Sample test of MP00612-4

  For the PBMC supernatant cultured for 5 days, the mean human PD-1/CD279 concentration was determined to be 382.5 pg/mL in PHA stimulated PBMC supernatant.

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  Sample test of MP00612-4

  Urine of eight individual healthy human was measured. The PD-1/CD279 concentration of detected samples was determined to be 856.9 pg/mL with a range of 280.3 - 1,536.5 pg/mL.

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  Sample test of MP00612-4

  Serum of sixteen individual healthy human donors was measured. The human PD-1/CD279 concentration of detected samples was determined to be 1,025.5 pg/mL with a range of 265.2 - 2,943.1 pg/mL.

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  Cytometric bead array standard curve of MP00612-2

  Cytometric bead array standard curve of MP00612-2, PD-1/CD279 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83632-2-PBS. Detection antibody: 83632-4-PBS. Standard: Eg0974. Range: 0.313-40 ng/mL.

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Product Information

83632-4-PBS targets PD-1/CD279 as part of a matched antibody pair:

MP00612-2: 83632-2-PBS capture and 83632-4-PBS detection (validated in Cytometric bead array)

MP00612-4: 83632-5-PBS capture and 83632-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Fusion Protein
• Full Name: programmed cell death 1
• Calculated Molecular Weight: 288 aa, 32 kDa
• GenBank Accession Number: BC074740
• Gene Symbol: PD-1
• Gene ID (NCBI): 5133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.