PCYT1B Polyclonal antibody

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  WB analysis of human placenta using 13765-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 13765-1-AP (PCYT1B antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13765-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13765-1-AP (PCYT1B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of human placenta using 13765-1-AP

  IP result of anti-PCYT1B (IP:13765-1-AP, 4ug; Detection:13765-1-AP 1:500) with human placenta tissue lysate 4000ug.

PCYT1B Polyclonal Antibody for WB, IP, ELISA

Cat No. 13765-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, ELISA

Synonyms

CCT B, CCT beta, CCTB, CCT-beta, Choline-phosphate cytidylyltransferase B

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Tested Applications

• Positive WB detected in: human placenta tissue, human brain tissue
• Positive IP detected in: human placenta tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

13765-1-AP targets PCYT1B in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCYT1B fusion protein Ag4748
• Full Name: phosphate cytidylyltransferase 1, choline, beta
• Calculated Molecular Weight: 351 aa, 40 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC045634
• Gene Symbol: PCYT1B
• Gene ID (NCBI): 9468
• RRID: AB_10642953
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5K3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCYT1B (Phosphate Cytidylyltransferase 1B, Choline), also named as CTβ, CCTB and CCT-Beta, is encoded by the gene belongs to the cytidylyltransferase family. It is involved in the regulation of phosphatidylcholine biosynthesis. Several alternatively spliced transcript variants encoding different isoforms have been found for this gene. PCYT1B encodes three isoforms of CTβ (CTβ1, CTβ2 and CTβ3). CTβ1, CTβ2 isoforms are present in humans and CTβ2 and CTβ3 isoforms have been identified in mice. The CTβ2 can be detected as 41 kDa, and CTβ3 can be detected as 39 kDa.

Publications

Species	Application	Title
mouse	WB	Neurobiol Dis; Choline metabolism in regulating inflammatory bowel disease-linked anxiety disorders: A multi-omics exploration of the gut-brain axis; Authors - Fan Zhang