PCTAIRE3 Polyclonal antibody

PCTAIRE3 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 12207-1-AP

Synonyms

PCTK3, PCTAIRE-motif protein kinase 3, PCTAIRE, EC:2.7.11.22, Cyclin-dependent kinase 18

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Validation Data Gallery

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  WB analysis of HEK-293 using 12207-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12207-1-AP (PCTAIRE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 12207-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 12207-1-AP (PCTAIRE3 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 12207-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12207-1-AP (PCTAIRE3 antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human ovary cancer using 12207-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12207-1-AP (PCTAIRE3 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HEK-293 using 12207-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using 12207-1-AP (PCTAIRE3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue
• Positive IHC detected in: human ovary cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

12207-1-AP targets PCTAIRE3 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCTAIRE3 fusion protein Ag2851
• Full Name: PCTAIRE protein kinase 3
• Calculated Molecular Weight: 472 aa, 54 kDa
• Observed Molecular Weight: 54 kDa, 58 kDa
• GenBank Accession Number: BC011526
• Gene Symbol: PCTAIRE3
• Gene ID (NCBI): 5129
• RRID: AB_906375
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCTAIRE3 (PCTAIRE-motif protein kinase 3), also named CDK18 or PCTK3, is a member of PCTAIRE subfamily. The PCTAIRE kinase (PCTK) subfamily of CDKs includes three members, PCTK1/CDK16, PCTK2/CDK17, and PCTK3/CDK18, which contain the PCTAIRE sequence instead of the PSTAIRE sequence (PMID: 24831015). PCTK3 has three isoforms with the molecular mass of 54-58kD.