PCSK9 Recombinant antibody

PCSK9 Uni-rAb^TM Recombinant Antibody for IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

230536E4

Cat no : 83004-4-RR

Synonyms

Narc1, HCHOLA3, FH3, AI747682, AI415265

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Validation Data Gallery

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  IF Staining of mouse liver using 83004-4-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse liver using 83004-4-RR

  Immunofluorescent analysis of (4% PFA) fixed mouse liver tissue using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• 

  IF Staining of HepG2 using 83004-4-RR

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCSK9 antibody (83004-4-RR, Clone: 230536E4 ) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  FC experiment of HeLa using 83004-4-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PCSK9 Recombinant antibody (83004-4-RR, Clone:230536E4) and PE-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83004-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83004-4-RR against Mouse PCSK9 were performed. The affinity constant is 384 nM.

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Tested Applications

• Positive IF-P detected in: mouse liver tissue
• Positive IF/ICC detected in: HepG2 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83004-4-RR targets PCSK9 in IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Recombinant protein
• Full Name: proprotein convertase subtilisin/kexin type 9
• Calculated Molecular Weight: 75KD
• GenBank Accession Number: NM_153565.2
• Gene Symbol: Pcsk9
• Gene ID (NCBI): 100102
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCSK9 (Proprotein convertase subtilisin/kexin type 9) is the ninth member of the proprotein convertase family that regulates lipoprotein homeostasis and altered PCSK9 expression was reportedly associated with tumor development and progression. PCSK9 is primarily synthesized and expressed in the liver but also in many other tissues, such as the intestinal tract, kidney, brain, heart, and blood vessels (PMID: 36242053).