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  • KD/KO Validated

PCSK9 Polyclonal antibody

PCSK9 Polyclonal Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA, IF

Conjugate

Unconjugated

Cat no : 27882-1-AP

Synonyms

NARC1, NARC 1, LDLCQ1, HCHOLA3, FH3



Tested Applications

Positive WB detected inHepG2 cells, rat liver tissue, mouse liver tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27882-1-AP targets PCSK9 in WB, IHC, FC (Intra), ELISA, IF applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCSK9 fusion protein Ag27254
Full Name proprotein convertase subtilisin/kexin type 9
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 72-78 kDa, 62 kDa
GenBank Accession NumberNM_174936.3
Gene Symbol PCSK9
Gene ID (NCBI) 255738
RRIDAB_2918134
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Proprotein convertase subtilisin/kexin type 9 (PCSK9) is a crucial protein governing the circulating levels of low density lipoprotein-cholesterol (LDL-C), by virtue of its pivotal role in the degradation of the LDL receptor (LDLR). PCSK9 is expressed in the kidney and lung. It is synthesized as a 72 kDa immature precursor that undergoes autocatalytic cleavage in the endoplasmic reticulum to generate a 63 kDa mature protein. The cleaved N-terminal fragment remains associated with the mature protein and is necessary for its secretion, allowing it to circulate in the blood. The ability of PCSK9 to regulate a diverse group of cell-surface proteins hinted that it might also be able to influence additional membrane proteins that are important in anti-tumour immune responses. Targeting PCSK9 to treat cancer is also attractive because two neutralizing antibodies against it, evolocumab and alirocumab, have already been approved for human clinical use to lower cholesterol levels. (PMID: 30522786, PMID: 22493497)

Protocols

Product Specific Protocols
WB protocol for PCSK9 antibody 27882-1-APDownload protocol
FC protocol for PCSK9 antibody 27882-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratIHC

Biochimie

High-salt diet induces dyslipidemia through the SREBP2/PCSK9 pathway in dahl salt-sensitive rats

Authors - Ya-Nan Ou-Yang
mouseWB

bioRxiv

Effect of Total Sphingomyelin Synthase Activity on Low Density Lipoprotein Catabolism in Mice

Authors - Zhiqiang Li
human,mouseWB,IHC,IF

iScience

Targeting PCSK9 reduces cancer cell stemness and enhances antitumor immunity in head and neck cancer

Authors - Qi-Chao Yang
  • KD Validated
human,mouseWB

FASEB J

Exogenous hydrogen sulfide alleviates hepatic endoplasmic reticulum stress via SIRT1/FoxO1/PCSK9 pathway in NAFLD

Authors - Xiaomeng Cui
humanWB,IF

JACC Basic Transl Sci

PCSK9 Promotes Hypoxia-Induced EC Pyroptosis by Regulating Smac Mitochondrion-Cytoplasm Translocation in Critical Limb Ischemia

Authors - Meixin Zhang
humanWB,IHC

Oncol Lett

PCSK9 promotes tumor cell proliferation and migration by facilitating CCL25 secretion in esophageal squamous cell carcinoma

Authors - Haijun Wang