PCSK7 Polyclonal antibody

PCSK7 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 12044-1-AP

Synonyms

SPC7, PC8, PC7, LPC, hPC8

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Validation Data Gallery

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  WB analysis using 12044-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12044-1-AP (PCSK7 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human lymphoma using 12044-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12044-1-AP (PCSK7 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human lymphoma using 12044-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 12044-1-AP (PCSK7 Antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: mouse spleen tissue, mouse thymus tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

12044-1-AP targets PCSK7 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCSK7 fusion protein Ag2674
• Full Name: proprotein convertase subtilisin/kexin type 7
• Calculated Molecular Weight: 785 aa, 86 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC010696
• Gene Symbol: PCSK7
• Gene ID (NCBI): 9159
• RRID: AB_2877820
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCSK7(Proprotein convertase subtilisin/kexin type 7) is also named as LPC, PC7, PC8, SPC7 and belongs to the peptidase S8 family. It has ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RXXX[KR]R consensus motif. The full length protein has 4 glycosylation sites and it can be N-glycosylated.

Publications

Species	Application	Title
human	IHC	Front Pharmacol; Therapeutic potential of the Proprotein Convertase Subtilisin/Kexin family in vascular disease; Authors - Bianca E. Suur