PCSK5 Polyclonal antibody

• Featured Product
• KD/KO Validated

PCSK5 Polyclonal Antibody for IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 16470-1-AP

Synonyms

hPC6, PC5, PC6, PC6A, PCSK5, Proprotein convertase 5, Proprotein convertase 6, SPC6

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Validation Data Gallery

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  IHC staining of human kidney using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16470-1-AP (PCSK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16470-1-AP (PCSK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16470-1-AP (PCSK5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16470-1-AP (PCSK5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human spleen using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human brain using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16470-1-AP (PC6 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16470-1-AP (PC6 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skin using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16470-1-AP (PC6 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 16470-1-AP (PC6 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human testis using 16470-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 16470-1-AP (PC6 antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive IHC detected in: human kidney tissue, human brain tissue, human skin tissue, human heart tissue, human testis tissue, human spleen tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

16470-1-AP targets PCSK5 in IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCSK5 fusion protein Ag9552
• Full Name: proprotein convertase subtilisin/kexin type 5
• Calculated Molecular Weight: 102 kDa, 207 kDa
• Observed Molecular Weight: 210 kDa
• GenBank Accession Number: BC012064
• Gene Symbol: PCSK5
• Gene ID (NCBI): 5125
• RRID: AB_2283381
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PC5 ,a mammalian kex2-like processing endoprotease, mediates posttranslational endoproteolytic processing for several integrin alpha subunits.It has been identified in rat and mouse endocrine and neuroendocrine tissues,and is encoded by multiple mRNAs that generate two splice variant isoforms referred to as PC5A (102KDa) and PC5B (207KDa) .PC5A is smaller than PC5B and it can be processed into two forms (117 kDa and 65 kDa) in neuronal cells(PMID:11457520).

Publications

Species	Application	Title
rat		Mol Med Rep; MicroRNA‑338‑3p regulates age‑associated osteoporosis via targeting PCSK5.; Authors - Jie Tong ; KD Validated
human		Biochem Biophys Rep; Secreted factors from adipose tissue-derived mesenchymal stem cells suppress oxygen/glucose deprivation-induced cardiomyocyte cell death via furin/PCSK-like enzyme activity.; Authors - Yuki Tachida
human	IHC	Mol Cell Biochem; PCSK5 downregulation promotes the inhibitory effect of andrographolide on glioblastoma through regulating STAT3; Authors - Huiyuan Gong
human	IF	Circ Heart Fail; Overexpression of ATP5F1A in Cardiomyocytes Promotes Cardiac Reverse Remodeling; Authors - Mengda Xu