PCSK2 Polyclonal antibody

PCSK2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10553-1-AP

Synonyms

KEX2 like endoprotease 2, NEC 2, NEC2, Neuroendocrine convertase 2, PC2, PCSK2, Prohormone convertase 2, Proprotein convertase 2, SPC2

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Validation Data Gallery

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  WB analysis using 10553-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10553-1-AP

  mouse brain tissue lysates were subjected to SDS PAGE followed by western blot with 10553-1-AP (PCSK2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below
• IF: See 1 publications below

Product Information

10553-1-AP targets PCSK2 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCSK2 fusion protein Ag0852
• Full Name: proprotein convertase subtilisin/kexin type 2
• Calculated Molecular Weight: 70.5 kDa
• Observed Molecular Weight: 65-75 kDa
• GenBank Accession Number: BC005815
• Gene Symbol: PCSK2
• Gene ID (NCBI): 5126
• RRID: AB_2299124
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCSK2(Prohormone convertase 2) is also named as NEC2(neuroendocrine convertase 2), PC2 and belongs to the peptidase S8 family. It is involved in the processing of hormone and other protein precursors at sites comprised of pairs of basic amino acid residues. PCSK2 is responsible for the release of glucagon from proglucagon in pancreatic A cells. This protein has 3 isoforms produced by alternative splicing. PCSK2 is biosynthesized as a 74-kDa inactive precursor which is matured to a 64-kDa active enzyme by autocatalytic removal of its prodomain (PMID: 8397508, 21092739)

Publications

Species	Application	Title
human	IF	Nat Commun; Functional anterior pituitary generated in self-organizing culture of human embryonic stem cells.; Authors - Chikafumi Ozone
rat	WB	J Cell Biol; VAMP4 regulates insulin levels by targeting secretory granules to lysosomes; Authors - Min Li
mouse	WB	Biochem Biophys Res Commun; Elongator regulates the melanocortin satiety pathway.; Authors - Joseph Walters
rat	WB	Function (Oxf); ER Redox Homeostasis Regulates Proinsulin Trafficking and Insulin Granule Formation in the Pancreatic Islet β-Cell; Authors - Kristen E Rohli
		iScience; Insulin receptor isoform B is required for efficient proinsulin processing in pancreatic β cells; Authors - Mingchao Jiang