PCNA Polyclonal antibody

PCNA Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA

Conjugate

Unconjugated

Publications

27

Cat no : 24036-1-AP

Synonyms

proliferating cell nuclear antigen, Cyclin

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Validation Data Gallery

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  WB analysis of HEK-293 using 24036-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 24036-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:7000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 24036-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse testis using 24036-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 24036-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 24036-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 24036-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 24036-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 24036-1-AP (PCNA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 24036-1-AP

  IP result of anti-PCNA (IP:24036-1-AP, 4ug; Detection:24036-1-AP 1:1000) with HEK-293 cells lysate 2000ug.

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  IHC staining of human gliomas using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24036-1-AP (PCNA antibody) at dilution of 1:1600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 40x lens).

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  IHC staining of human stomach cancer using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24036-1-AP (PCNA antibody) at dilution of 1:1200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 10x lens).

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  IHC staining of human stomach cancer using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 40x lens).

• 

  IHC staining of human breast cancer using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 24036-1-AP (PCNA Antibody) at dilution of 1:400 (under 10x lens).

• 

  IHC staining of human gliomas using 24036-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 24036-1-AP (PCNA antibody) at dilution of 1:1600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human breast cancer using 24036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 24036-1-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 24036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using 24036-1-AP (PCNA antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 24036-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 24036-1-AP (PCNA antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, Raji cells, HEK-293 cells, NIH/3T3 cells, MCF-7 cells, rat liver tissue, HepG2 cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human breast cancer tissue, human gliomas tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: human breast cancer tissue
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:14000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 14 publications below
• IHC: See 11 publications below
• IF: See 7 publications below
• RIP: See 1 publications below

Product Information

24036-1-AP targets PCNA in WB, IHC, IF/ICC, IF-P, IP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCNA fusion protein Ag7416
• Full Name: proliferating cell nuclear antigen
• Calculated Molecular Weight: 29 kDa/31 kDa
• Observed Molecular Weight: 36-38 kDa
• GenBank Accession Number: BC000491
• Gene Symbol: PCNA
• Gene ID (NCBI): 5111
• RRID: AB_2879414
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The proliferating cell nuclear antigen (PCNA), a protein synthesized in early G1 and S phases of the cell cycle, functions in cell cycle progression, DNA replication and DNA repair. In early S phase, PCNA exhibits granular distribution and is absent from the nucleoli; however, in late S phase, it relocates to the nucleoli. PCNA exists in two basic forms: one involved in ongoing DNA replication, which localizes specifically to the nucleus, and a second, soluble form, not implicated in constant synthesis. Interestingly, the latter form degrades in the presence of organic solvents, rendering it undetectable by histological methods in tissues using organic fixatives, and thus also providing a method of visualizing only the synthesizing form.This antibody specifically react with the 36kd human PCNA protein.

Publications

Species	Application	Title
mouse	IHC	Int J Biol Sci; Tendon Cells Root Into (Instead of Attach to) Humeral Bone Head via Fibrocartilage-Enthesis; Authors - Zheng Wang
rat	WB,IF	Biochem Pharmacol; Plasma Exosomes Confer Hypoxic Pulmonary Hypertension by Transferring LOX-1 Cargo to Trigger Phenotypic Switching of Pulmonary Artery Smooth Muscle Cells; Authors - Ning Huang
rat	WB	FASEB J; S1P transporter SPNS2 regulates proper postnatal retinal morphogenesis.; Authors - Chao Fang
rat	WB,IHC	Biochem Pharmacol; CGRP attenuates pulmonary vascular remodeling by inhibiting the cGAS-STING-NFκB pathway in pulmonary arterial hypertension; Authors - Xin Yan
human	WB	Am J Cancer Res; YY1-induced lncRNA00511 promotes melanoma progression via the miR-150-5p/ADAM19 axis; Authors - Ya-Ni Chen
mouse	WB	Hepatol Commun; Hepatocellular carcinoma cells downregulate NADH:Ubiquinone Oxidoreductase Subunit B3 to maintain reactive oxygen species homeostasis; Authors - Zhendong Zhang