PCK2 Monoclonal antibody

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  WB analysis using 67676-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 67676-1-Ig

  rat liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of pig liver using 67676-1-Ig

  pig liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of rabbit liver using 67676-1-Ig

  rabbit liver tissue were subjected to SDS PAGE followed by western blot with 67676-1-Ig (PCK2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 67676-1-Ig

  IP result of anti-PCK2 (IP:67676-1-Ig, 4ug; Detection:67676-1-Ig 1:2000) with HepG2 cells lysate 1640 ug.

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  IHC staining of human lung cancer using 67676-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 67676-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 67676-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 67676-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67676-1-Ig (PCK2 antibody) at dilution of 1:8000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

PCK2 Monoclonal Antibody for WB, IHC, IP, ELISA

Cat No. 67676-1-Ig

Publications(3)

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat, pig, rabbit

Applications

WB, IHC, IP, ELISA

Synonyms

Phosphoenolpyruvate carboxykinase 2, mitochondrial, PEPCK-M, PEPCK2, mtPCK2, EC:4.1.1.32

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

CoraLite® Plus 488

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Tested Applications

• Positive WB detected in: HuH-7 cells, rat liver tissue, pig liver tissue, rabbit liver tissue, HepG2 cells, LNCaP cells, K562 cells, Neuro-2a cells, HSC-T6 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human lung cancer tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:4000-1:16000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 3 publications below

Product Information

67676-1-Ig targets PCK2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig, rabbit samples.

• Tested Reactivity: human, mouse, rat, pig, rabbit
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PCK2 fusion protein Ag7021
• Full Name: phosphoenolpyruvate carboxykinase 2 (mitochondrial)
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC001454
• Gene Symbol: PCK2
• Gene ID (NCBI): 5106
• RRID: AB_2882871
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q16822
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis. Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa.

Publications

Species	Application	Title
rat	WB	Food Funct; Black bean husk and black rice anthocyanin extracts modulated gut microbiota and serum metabolites for improvement in type 2 diabetic rats.; Authors - Mubai Sun
human,mouse	WB	Front Pharmacol; Berberrubine, a Main Metabolite of Berberine, Alleviates Non-Alcoholic Fatty Liver Disease via Modulating Glucose and Lipid Metabolism and Restoring Gut Microbiota; Authors - Sa Yang
rat	WB	Heliyon; Tanshinone IIA attenuates fluoride-induced spinal cord injury by inhibiting ferroptosis and inflammation; Authors - Qingfeng Shen