Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue, mouse liver tissue |
| Positive IHC detected in | human breast cancer tissue, mouse kidney tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
| Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:100-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14892-1-AP targets PCK1/2 in WB, IHC, IF/ICC, FC (Intra), CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6681 Product name: Recombinant human PCK2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 292-640 aa of BC001454 Sequence: AGKKRYVAAAFPSACGKTNLAMMRPALPGWKVECVGDDIAWMRFDSEGRLRAINPENGFFGVAPGTSATTNPNAMATIQSNTIFTNVAETSDGGVYWEGIDQPLPPGVTVTSWLGKPWKPGDKEPCAHPNSRFCAPARQCPIMDPAWEAPEGVPIDAIIFGGRRPKGVPLVYEAFNWRHGVFVGSAMRSESTAAAEHKGKIIMHDPFAMRPFFGYNFGHYLEHWLSMEGRKGAQLPRIFHVNWFRRDEAGHFLWPGFGENARVLDWICRRLEGEDSARETPIGLVPKEGALDLSGLRAIDTTQLFSLPKDFWEQEVRDIRSYLTEQVNQDLPKEVLAELEALERRVHKM Predict reactive species |
| Full Name | phosphoenolpyruvate carboxykinase 2 (mitochondrial) |
| Calculated Molecular Weight | 71 kDa |
| Observed Molecular Weight | 66-71 kDa |
| GenBank Accession Number | BC001454 |
| Gene Symbol | PCK2 |
| Gene ID (NCBI) | 5106 |
| RRID | AB_2160044 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q16822 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCK2(phosphoenolpyruvate carboxykinase [GTP], mitochondrial) is also named as PEPCK2, PEPCK-M and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyses the irreversible conversion of oxaloacetate to phosphoenolpyruvate, an important initial step in hepatic gluconeogenesis.Defects in PCK2 are the cause of mitochondrial phosphoenolpyruvate carboxykinase deficiency (M-PEPCKD). It has 2 isoforms produced by alternative splicing with the molecular weight of 71 kDa and 48 kDa. This antibody has weak cross-reactivity with PCK1 protein due to high sequence homology between PCK1 and PCK2.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for PCK1/2 antibody 14892-1-AP | Download protocol |
| IF protocol for PCK1/2 antibody 14892-1-AP | Download protocol |
| IHC protocol for PCK1/2 antibody 14892-1-AP | Download protocol |
| WB protocol for PCK1/2 antibody 14892-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-PCK2 (cat. #14892-1-AP) has 45 citations published in journals such as Science Advances, Cell Death & Disease, EBioMedicine, Diabetologia, Journal of Biological Chemistry, Cell Signaling, and Biochemical & Biophysical Research Communications. Associated research fields include metabolic reprogramming and gluconeogenesis, diabetes and insulin resistance, oncology (hepatic, gastric, lung, glioblastoma, medulloblastoma), ferroptosis, mitochondrial biology, NAFLD/NASH, stroke, and preeclampsia.
| Species | Application | Title |
|---|---|---|
Sci Adv Atf3-mediated metabolic reprogramming in hepatic macrophage orchestrates metabolic dysfunction-associated steatohepatitis | ||
Cell Death Dis CHCHD2 and CHCHD10 regulate mitochondrial dynamics and integrated stress response. | ||
Cell Death Dis 18F-FDG-PET/CT-negative gastric cancer employs glutamine-based gluconeogenesis and fatty acid oxidation to support tumor growth. | ||
EBioMedicine Homozygous variant in translocase of outer mitochondrial membrane 7 leads to metabolic reprogramming and microcephalic osteodysplastic dwarfism with moyamoya disease | ||
Diabetologia Targeting BCAA metabolism to potentiate metformin's therapeutic efficacy in the treatment of diabetes in mice | ||
J Transl Med Dihydromyricetin ameliorates hepatic steatosis and insulin resistance via AMPK/PGC-1α and PPARα-mediated autophagy pathway |
Reviews
What customers say
The single review for 14892-1-AP is a 5-star rating from Kristian Laursen (June 2021). The reviewer used the antibody at a 1:200 dilution for immunofluorescence on transgenic mouse kidney tissue (TRACK). They reported successful detection of PCK2 (red) in proximal tubules where HIF1A signaling is activated. Overall, the feedback is positive, confirming reliable performance in IF applications on mouse tissue.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kristian (Verified Customer) (06-07-2021) | PCK2 (red) is detected in proximale tubules where HIF1A-signaling is activated.
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