PCK1 Monoclonal antibody, PBS Only

PCK1 Monoclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat, Pig

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

2F11B5

Cat no : 66862-1-PBS

Synonyms

PCK1, PEPCK C, PEPCK1, PEPCKC

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Validation Data Gallery

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  WB analysis using 66862-1-Ig (same clone as 66862-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66862-1-Ig (PCK1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse kidney using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human kidney using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human colon using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66862-1-Ig (PCK1 antibody) at dilution of 1:32000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66862-1-Ig (same clone as 66862-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PCK1 antibody (66862-1-Ig, Clone: 2F11B5 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66862-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66862-1-PBS targets PCK1 in WB, IF, IHC, ELISA applications and shows reactivity with Human, Mouse, Rat, Pig samples.

• Tested Reactivity: Human, Mouse, Rat, Pig
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PCK1 fusion protein Ag9720
• Full Name: phosphoenolpyruvate carboxykinase 1 (soluble)
• Calculated Molecular Weight: 622 aa, 69 kDa
• Observed Molecular Weight: 65-69 kDa
• GenBank Accession Number: BC023978
• Gene Symbol: PCK1
• Gene ID (NCBI): 5105
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).