|Positive WB detected in||mouse liver tissue, HepG2 cells, rat liver tissue|
|Positive IP detected in||HepG2 cells|
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunofluorescence (IF)||IF : 1:200-1:800|
|Sample-dependent, check data in validation data gallery|
16754-1-AP targets PCK1 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse, rat, goat, pig, bovine|
|Host / Isotype||Rabbit / IgG|
|Immunogen||PCK1 fusion protein Ag10261|
|Full Name||phosphoenolpyruvate carboxykinase 1 (soluble)|
|Calculated molecular weight||622 aa, 69 kDa|
|Observed molecular weight||66-69 kDa|
|GenBank accession number||BC023978|
|Gene ID (NCBI)||5105|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.
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The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
James (Verified Customer) (11-18-2022)
Detection of PCK1 in liver protein extracts