PCK1 Polyclonal antibody

• Featured Product
• KD/KO Validated

PCK1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

71

Reviews (1)

Cat no : 16754-1-AP

Synonyms

Serine-protein kinase PCK1, PEPCK-C, PEPCK1, EC:4.1.1.32, EC:2.7.11.-

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Validation Data Gallery

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  WB analysis using 16754-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16754-1-AP (PCK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16754-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16754-1-AP (PCK1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 16754-1-AP

  IP result of anti-PCK1 (IP:16754-1-AP, 4ug; Detection:16754-1-AP 1:500) with HepG2 cells lysate 3600ug.

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  IF Staining of HepG2 using 16754-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using PCK1 antibody (16754-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue, HepG2 cells, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 62 publications below
• IHC: See 5 publications below
• IF: See 11 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Product Information

16754-1-AP targets PCK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, pig, bovine, goat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCK1 fusion protein Ag10261
• Full Name: phosphoenolpyruvate carboxykinase 1 (soluble)
• Calculated Molecular Weight: 622 aa, 69 kDa
• Observed Molecular Weight: 66-69 kDa
• GenBank Accession Number: BC023978
• Gene Symbol: PCK1
• Gene ID (NCBI): 5105
• RRID: AB_2160031
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).

Publications

Species	Application	Title
human	WB,IP	Nature; The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.; Authors - Daqian Xu ; KD Validated
mouse	WB	Nat Commun; Glycogen metabolism regulates macrophage-mediated acute inflammatory responses.; Authors - Jingwei Ma
mouse	IF	Adv Healthc Mater; Mesenchymal Stem Cell Exosomes Encapsulated Oral Microcapsules for Acute Colitis Treatment.; Authors - Jingjing Gan
human	WB	Biomaterials; Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance.; Authors - Meiling Wu
mouse	WB	Proc Natl Acad Sci U S A; Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver.; Authors - Daniel Mauvoisin ; KO Validated
mouse	WB	Diabetes; Prostaglandin F2α Facilitates Hepatic Glucose Production Through CaMKIIγ/p38/FoxO1-Signaling Pathway in Fasting and Obesity.; Authors - Yuanyang Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

James (Verified Customer) (11-18-2022)

Detection of PCK1 in liver protein extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Liver

PCK1 Antibody Western Blot validation (1/1000 dilution) in Liver (Cat no:16754-1-AP)