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PCK1 Polyclonal antibody
PCK1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 16754-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, HepG2 cells, rat liver tissue |
Positive IP detected in | HepG2 cells |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 5 publications below |
WB | See 61 publications below |
IHC | See 5 publications below |
IF | See 11 publications below |
IP | See 3 publications below |
CoIP | See 1 publications below |
Product Information
16754-1-AP targets PCK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, pig, bovine, goat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | PCK1 fusion protein Ag10261 |
Full Name | phosphoenolpyruvate carboxykinase 1 (soluble) |
Calculated Molecular Weight | 622 aa, 69 kDa |
Observed Molecular Weight | 66-69 kDa |
GenBank Accession Number | BC023978 |
Gene Symbol | PCK1 |
Gene ID (NCBI) | 5105 |
RRID | AB_2160031 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
PCK1(Phosphoenolpyruvate carboxykinase, cytosolic) is also named as PEPCK1 and belongs to the phosphoenolpyruvate carboxykinase [GTP] family. It catalyzes the formation of phosphoenolpyruvate from oxaloacetate, with the release of carbon dioxide and GDP. It is also a main control point for the regulation of gluconeogenesis. In eukaryotes there are two isozymes: a cytoplasmic one and a mitochondrial one. Defects in PCK1 are the cause of cytosolic phosphoenolpyruvate carboxykinase deficiency (C-PEPCKD).
Protocols
Product Specific Protocols | |
---|---|
WB protocol for PCK1 antibody 16754-1-AP | Download protocol |
IF protocol for PCK1 antibody 16754-1-AP | Download protocol |
IP protocol for PCK1 antibody 16754-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nature The gluconeogenic enzyme PCK1 phosphorylates INSIG1/2 for lipogenesis.
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Nat Commun Glycogen metabolism regulates macrophage-mediated acute inflammatory responses. | ||
Adv Healthc Mater Mesenchymal Stem Cell Exosomes Encapsulated Oral Microcapsules for Acute Colitis Treatment. | ||
Biomaterials Liver-targeted Nano-MitoPBN normalizes glucose metabolism by improving mitochondrial redox balance. | ||
Proc Natl Acad Sci U S A Circadian clock-dependent and -independent rhythmic proteomes implement distinct diurnal functions in mouse liver.
| ||
Diabetes Prostaglandin F2α Facilitates Hepatic Glucose Production Through CaMKIIγ/p38/FoxO1-Signaling Pathway in Fasting and Obesity. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH James (Verified Customer) (11-18-2022) | Detection of PCK1 in liver protein extracts
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