PCIF1 Polyclonal antibody

PCIF1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 16082-1-AP

Synonyms

C20orf67, PCIF1



Tested Applications

Positive WB detected inRamos cells, HepG2 cells, Jurkat cells, HEK-293T cells
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16082-1-AP targets PCIF1 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PCIF1 fusion protein Ag9080
Full Name PDX1 C-terminal inhibiting factor 1
Calculated Molecular Weight 704 aa, 81 kDa
Observed Molecular Weight 81 kDa
GenBank Accession NumberBC013365
Gene Symbol PCIF1
Gene ID (NCBI) 63935
RRIDAB_2159996
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for PCIF1 antibody 16082-1-APDownload protocol
IHC protocol for PCIF1 antibody 16082-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human,mouseWB,IP

Mol Cell

RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits

Authors - Yuanjun Li
humanWB

Nat Commun

HIV reprograms host m 6 Am RNA methylome by viral Vpr protein-mediated degradation of PCIF1

Authors - Qiong Zhang
pigWB

Cell Rep

Alphaherpesvirus US3 protein-mediated inhibition of the m6A mRNA methyltransferase complex.

Authors - Robert J J Jansens
human,mouseWB,IF

EMBO J

Role of PCIF1-mediated 5\'-cap N6-methyladeonsine mRNA methylation in colorectal cancer and anti-PD-1 immunotherapy

Authors - Lingling Wang
humanWB

Proc Natl Acad Sci U S A

PCIF1-mediated deposition of 5'-cap N6,2'-O-dimethyladenosine in ACE2 and TMPRSS2 mRNA regulates susceptibility to SARS-CoV-2 infection

Authors - Lingling Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Anirban (Verified Customer) (01-25-2023)

blocking 1 hr in 5% milk/TBST. Primary ab- 1/1000 in 3% BSA/TBST at 4 deg for 16 hrs, Secondary ab-1/5000 in 5% milk/TBST.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: 786-O, 769p
PCIF1 Antibody Western Blot validation (1/1000 dilution) in 786-O, 769p (Cat no:16082-1-AP)