• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

PCF11 Recombinant antibody, PBS Only

PCF11 Uni-rAbTM Recombinant Antibody for IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

241213B12

Cat no : 84081-5-PBS

Synonyms

Pre-mRNA cleavage complex II protein Pcf11, Pre-mRNA cleavage complex 2 protein Pcf11, KIAA0824, 241213B12

Validation Data Gallery

Filter:
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using PCF11 Recombinant antibody (84081-5-RR)

    IF Staining of HeLa using 84081-5-RR (same clone as 84081-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PCF11 antibody (84081-5-RR, Clone: 241213B12 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84081-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-2 OS using PCF11 Recombinant antibody (84081-5-RR)

    FC experiment of U-2 OS using 84081-5-RR (same clone as 84081-5-PBS)

    1x10^6 U-2 OS were intracellularly stained with 0.25 ug PCF11 Recombinant antibody (84081-5-RR, Clone:241213B12) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 84081-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84081-5-RR

    Affinity and Kinetic Characterization of 84081-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84081-5-RR against Human PCF11 were performed. The affinity constant is 9.47 nM.

Product Information

The immunogen of 84081-5-PBS is PCF11 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen PCF11 fusion protein Ag20263
Full Name PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)
Calculated Molecular Weight 1555 aa, 173 kDa
GenBank Accession NumberBC146778
Gene Symbol PCF11
Gene ID (NCBI) 51585
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

In Saccharomyces cerevisiae, the cleavage/polyadenylation factor Pcf11 is a crucial regulatory factor required for recruiting polyadenylation machinery to elongating RNA polymerase II (RNAPII), and is necessary for correct transcriptional termination. Pcf11 (PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)), is a 1,555 amino acid nuclear protein that is a component of pre-mRNA cleavage complex II. It is suggested that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Pcf11 contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. The gene encoding Pcf11 is located on human chromosoem 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.