PCF11 Recombinant antibody

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  WB analysis using 84081-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84081-4-RR (PCF11 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  FC experiment of U-2 OS using 84081-4-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug PCF11 Recombinant antibody (84081-4-RR, Clone:241213F9) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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  Affinity and Kinetic Characterization of 84081-4-RR

  Biolayer interferometry (BLl) kinetic assays of 84081-4-RR against Human PCF11 were performed. The affinity constant is 0.998 nM.

PCF11 {ptg:Brand} Recombinant Antibody for WB, FC (Intra), ELISA

Cat No. 84081-4-RR

Clone No.241213F9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC (Intra), ELISA

Synonyms

Pre-mRNA cleavage complex II protein Pcf11, Pre-mRNA cleavage complex 2 protein Pcf11, KIAA0824, 241213F9

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293T cells, Jurkat cells, Neuro-2a cells, RAW 264.7 cells,
• Positive FC (Intra) detected in: U-2 OS

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84081-4-RR targets PCF11 in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: PCF11 fusion protein Ag20263
• Full Name: PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)
• Calculated Molecular Weight: 1555 aa, 173 kDa
• Observed Molecular Weight: 180~200 kDa
• GenBank Accession Number: BC146778
• Gene Symbol: PCF11
• Gene ID (NCBI): 51585
• RRID: AB_3671649
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: O94913
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In Saccharomyces cerevisiae, the cleavage/polyadenylation factor Pcf11 is a crucial regulatory factor required for recruiting polyadenylation machinery to elongating RNA polymerase II (RNAPII), and is necessary for correct transcriptional termination. Pcf11 (PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)), is a 1,555 amino acid nuclear protein that is a component of pre-mRNA cleavage complex II. It is suggested that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Pcf11 contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. The gene encoding Pcf11 is located on human chromosoem 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11.