PCF11 Polyclonal antibody

• Featured Product
• KD/KO Validated

PCF11 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IP, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 23540-1-AP

Synonyms

Pre-mRNA cleavage complex II protein Pcf11, Pre-mRNA cleavage complex 2 protein Pcf11, KIAA0824

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Validation Data Gallery

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  WB analysis of K-562 using 23540-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 23540-1-AP (PCF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 23540-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23540-1-AP (PCF11 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 23540-1-AP

  IP result of anti-PCF11 (IP:23540-1-AP, 4ug; Detection:23540-1-AP 1:1000) with K-562 cells lysate 2400 ug.

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  IHC staining of human intrahepatic cholangiocarcinoma using 23540-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 23540-1-AP (PCF11 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: K-562 cells, HeLa cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• CoIP: See 1 publications below

Product Information

23540-1-AP targets PCF11 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PCF11 fusion protein Ag20263
• Full Name: PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)
• Calculated Molecular Weight: 1555 aa, 173 kDa
• Observed Molecular Weight: 173 kDa
• GenBank Accession Number: BC146778
• Gene Symbol: PCF11
• Gene ID (NCBI): 51585
• RRID: AB_2879293
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

In Saccharomyces cerevisiae, the cleavage/polyadenylation factor Pcf11 is a crucial regulatory factor required for recruiting polyadenylation machinery to elongating RNA polymerase II (RNAPII), and is necessary for correct transcriptional termination. Pcf11 (PCF11, cleavage and polyadenylation factor subunit, homolog (S. cerevisiae)), is a 1,555 amino acid nuclear protein that is a component of pre-mRNA cleavage complex II. It is suggested that Pcf11 is capable of promoting the dissociation of Pol II elongation complexes from DNA. Pcf11 contains a CTD-interaction domain that binds in a phospho-dependent manner to the heptad repeats within the RNA polymerase II CTD. The gene encoding Pcf11 is located on human chromosoem 11, which houses over 1,400 genes and comprises nearly 4% of the human genome. Jervell and Lange-Nielsen syndrome, Jacobsen syndrome, Niemann-Pick disease, hereditary angioedema and Smith-Lemli-Opitz syndrome are associated with defects in genes that maps to chromosome 11. This antibody is specific to the 173kd human PCF11 protein.

Publications

Species	Application	Title
mouse	WB	Mol Cell; RNA Pol II preferentially regulates ribosomal protein expression by trapping disassociated subunits; Authors - Yuanjun Li
human	CoIP	Neuron; Suppression of premature transcription termination leads to reduced mRNA isoform diversity and neurodegeneration.; Authors - Geneva R LaForce
human	WB	Nucleic Acids Res; CRISPRpas: programmable regulation of alternative polyadenylation by dCas9.; Authors - Jihae Shin ; KD Validated
human	WB	Cell Rep; LINC00921 reduces lung cancer radiosensitivity by destabilizing NUDT21 and driving aberrant MED23 alternative polyadenylation; Authors - Nasha Zhang
human	WB	Biochimie; An investigation into the role of ATP in the mammalian pre-mRNA 3' cleavage reaction.; Authors - Asya Khleborodova