Progranulin/PGRN Polyclonal antibody

• Featured Product
• KD/KO Validated

Progranulin/PGRN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

13

Reviews (1)

Cat no : 18410-1-AP

Synonyms

acrogranin, GEP, GP88, granulin, Granulin A Granulin 5, Granulin B Granulin 4, Granulin C Granulin 6, Granulin D Granulin 7, Granulin E, Granulin F Granulin 3, Granulin G Granulin 2, Granulins, GRN, PCDGF, PEPI, PGRN, Proepithelin

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Validation Data Gallery

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  WB analysis using 18410-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 18410-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 18410-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 18410-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 18410-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18410-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 18410-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of THP-1 using 18410-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 18410-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 18410-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human saliva using 18410-1-AP

  human saliva were subjected to SDS PAGE followed by western blot with 18410-1-AP (Granulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 18410-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 18410-1-AP (Granulin antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, A431 cells, HeLa cells, HEK-293 cells, PC-3 cells, human saliva, mouse heart tissue, mouse lung tissue, rat brain tissue, U-937 cells, THP-1 cells, RAW 264.7 cells
• Positive IHC detected in: human pancreas cancer tissue, human kidney tissue, human lung cancer tissue, human ovary tumor tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Product Information

18410-1-AP targets Progranulin/PGRN in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Progranulin/PGRN fusion protein Ag13178
• Full Name: granulin
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC000324
• Gene Symbol: Granulin
• Gene ID (NCBI): 2896
• RRID: AB_10598161
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system.

Publications

Species	Application	Title
rat	WB	Transl Psychiatry; Progranulin improves neural development via the PI3K/Akt/GSK-3β pathway in the cerebellum of a VPA-induced rat model of ASD.; Authors - Lili Wang
human	IHC	J Proteome Res; Mining the gastric cancer secretome: identification of GRN as a potential diagnostic marker for early gastric cancer.; Authors - Loei Hendrick H
human	IF	J Mol Med (Berl); Progranulin alleviates podocyte injury via regulating CAMKK/AMPK-mediated autophagy under diabetic conditions.; Authors - Di Zhou
human	WB	Front Oncol; GRN is a prognostic biomarker and correlated with immune infiltration in glioma: A study based on TCGA data; Authors - Su-Mei Xu
rat	WB	Neuropharmacology; Abnormal spatiotemporal expression pattern of progranulin and neurodevelopment impairment in VPA-induced ASD rat model.; Authors - Junying Lan
rat	WB,IHC,IF	Int J Mol Sci; Involvement of Progranulin and Granulin Expression in Inflammatory Responses after Cerebral Ischemia.; Authors - Ichiro Horinokita

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Sharan (Verified Customer) (06-24-2020)

The antibody detects the GRN protein but there is also a lot of cross-reactivity with non-specific antigens in HeLa lysate

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa cells

Progranulin/PGRN Antibody Western Blot, validation (1:1000 dilution) in HeLa cells (Cat no:18410-1-AP)