Progranulin/PGRN Polyclonal antibody

Progranulin/PGRN Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 10053-1-AP

Synonyms

granulin, Granulin-1, Granulin precursor, Glycoprotein of 88 Kda, Glycoprotein 88

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Validation Data Gallery

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  WB analysis using 10053-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10053-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 10053-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10053-1-AP (Granulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human colon cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10053-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10053-1-AP (Granulin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A431 cells, HEK-293 cells, MCF-7 cells
• Positive IHC detected in: human colon cancer tissue, human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• IF: See 1 publications below

Product Information

10053-1-AP targets Progranulin/PGRN in WB, IHC, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Progranulin/PGRN fusion protein Ag0010
• Full Name: granulin
• Calculated Molecular Weight: 64 kDa
• Observed Molecular Weight: 64 kDa
• GenBank Accession Number: BC010577
• Gene Symbol: Granulin
• Gene ID (NCBI): 2896
• RRID: AB_514063
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

GRN, also known as PGRN or PCDGF, is a cysteine-rich protein of 68.5 kDa that is typically secreted into a highly glycosylated 88 kDa form. PGRN is a unique growth factor that plays an important role in cutaneous wound healing. It has an anti-inflammatory effect and promotes cell proliferation. When PCDGF is degraded to several 6-25 kDa fragments, called granulins (GRNs) by neutrophil proteases, a pro-inflammatory reaction occurs. PGRN is widely expressed, particularly in epithelial cells, immune cells, neurons, and chondrocytes. High levels of PGRN expression have been reported in human cancers, and its expression is closely correlated with the development and metastasis of several cancers. The recent discovery that mutations in the gene encoding for pro-granulin (GRN) cause frontotemporal lobar degeneration (FTLD), and other neurodegenerative diseases leading to dementia, has brought renewed interest in progranulin and its functions in the central nervous system. Several in vitro studies have revealed that PGRN is classified into two isoforms according to its glycosylation status, the glycosylated immature isoform (58-68 kDa) and the fully glycosylated mature secretory isoform (∼88 kDa) (PMID: 25838514).

Publications

Species	Application	Title
human	IHC	Chin Med J (Engl); Expression of PC cell-derived growth factor and vascular endothelial growth factor in esophageal squamous cell carcinoma and their clinicopathologic significance.; Authors - Chen Xiang-yu XY
	IF	Cell Prolif; m6 A promotes planarian regeneration; Authors - Guanshen Cui

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