PBX3 Polyclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

PBX3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12571-1-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of human lung using 12571-1-AP (same clone as 12571-1-PBS)

  human lung tissue were subjected to SDS PAGE followed by western blot with 12571-1-AP (PBX3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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  IP experiment of HL-60 using 12571-1-AP (same clone as 12571-1-PBS)

  IP result of anti-PBX3 (IP:12571-1-AP, 4ug; Detection:12571-1-AP 1:500) with HL-60 cells lysate 3040ug. This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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  IHC staining of human lung cancer using 12571-1-AP (same clone as 12571-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12571-1-AP (PBX3 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 12571-1-AP (same clone as 12571-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12571-1-AP (PBX3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12571-1-PBS in a different storage buffer formulation.

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Product Information

12571-1-PBS targets PBX3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PBX3 fusion protein Ag3239
• Full Name: pre-B-cell leukemia homeobox 3
• Calculated Molecular Weight: 434 aa, 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC016977
• Gene Symbol: PBX3
• Gene ID (NCBI): 5090
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PBX3, also named as Pre-B-cell leukemia transcription factor 3, is a 434 amino acid protein ,which contains 1 homeobox DNA-binding domain and belongs to the TALE/PBX homeobox family. PBX3 localizes in the nucleus and as a transcriptional activator that binds the sequence 5'-ATCAATCAA-3'. PBX3 is ubiquitously expressed in many tissues.