PBLD Polyclonal antibody

PBLD Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27891-1-AP

Synonyms

MAWBP, MAWD binding protein, MAWDBP, PBLD

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Validation Data Gallery

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  WB analysis of mouse kidney using 27891-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27891-1-AP (PBLD antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 27891-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 27891-1-AP (PBLD antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 27891-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 27891-1-AP (PBLD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 27891-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27891-1-AP (PBLD antibody) at dilution of 1:600 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 27891-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 27891-1-AP (PBLD antibody) at dilution of 1:600 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse liver tissue, mouse kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:300-1:1200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27891-1-AP targets PBLD in WB, IHC, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PBLD fusion protein Ag23591
• Full Name: phenazine biosynthesis-like protein domain containing
• Observed Molecular Weight: 30-32 kDa
• GenBank Accession Number: BC009738
• Gene Symbol: PBLD
• Gene ID (NCBI): 64081
• RRID: AB_2881007
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.