PAX6 Monoclonal antibody, PBS Only

PAX6 Monoclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2C5A1

Cat no : 67529-1-PBS

Synonyms

AN, AN2, Aniridia type II protein, D11S812E, MGDA, Oculorhombin, paired box 6, Paired box protein Pax 6, PAX6, WAGR

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Validation Data Gallery

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  WB analysis of rat retina using 67529-1-Ig (same clone as 67529-1-PBS)

  rat retina tissue were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67529-1-Ig (same clone as 67529-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67529-1-Ig (same clone as 67529-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of JAR using 67529-1-Ig (same clone as 67529-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

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  WB analysis of hTERT-RPE1 using 67529-1-Ig (same clone as 67529-1-PBS)

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67529-1-Ig (same clone as 67529-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Neuro-2a using 67529-1-Ig (same clone as 67529-1-PBS)

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 67529-1-Ig (PAX6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse eye using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse eye tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 67529-1-Ig (same clone as 67529-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67529-1-Ig (PAX6 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67529-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67529-1-PBS targets PAX6 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: PAX6 fusion protein Ag29977
• Full Name: paired box 6
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC011953
• Gene Symbol: PAX6
• Gene ID (NCBI): 5080
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PAX6, a paired domain and homeodomain-containing transcription factor. Interaction with TRIM11 leads to ubiquitination of PAX6 and its proteasomal degradation. PAX6 is one of the earliest genes expressed in the eye field and considered a master control gene for retinal and eye development. PAX6 also regulates the development of the olfactory, central nervous systems, pituitary, and pancreas. PAX6 mutations can cause complex ocular disorders such as aniridia and Peter's anomaly.