PAX3 Polyclonal antibody, PBS Only

PAX3 Polyclonal Antibody for WB, IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 51036-2-PBS

Synonyms

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Validation Data Gallery

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  WB analysis of mouse skin using 51036-2-AP (same clone as 51036-2-PBS)

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

• 

  WB analysis of A375 using 51036-2-AP (same clone as 51036-2-PBS)

  A375 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 51036-2-AP (same clone as 51036-2-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 51036-2-AP (same clone as 51036-2-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 51036-2-AP (PAX3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 51036-2-AP (same clone as 51036-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51036-2-AP (PAX3 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

• 

  IHC staining of rat brain using 51036-2-AP (same clone as 51036-2-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 51036-2-AP (PAX3 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 51036-2-PBS in a different storage buffer formulation.

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Product Information

51036-2-PBS targets PAX3 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAX3 fusion protein Ag0477
• Full Name: paired box 3
• Calculated Molecular Weight: 120 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC114363
• Gene Symbol: PAX3
• Gene ID (NCBI): 5077
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

PAX3, a transcription factor and multifunctional regulatory protein, is normally expressed during embryonic development. In the nervous system, PAX3 is involved in neural tube closure, neural crest development, and peripheral neuron differentiation. In the present study, PAX3 was reported as a novel regulator of GFAP transcription, and the overexpression and suppression of PAX3 could inhibit and promote NSC differentiation, respectively. In muscle development, PAX3 ensures the survival of myogenic progenitor cells with Pax3-expressing progenitors giving rise to both skeletal and smooth muscle cells. PAX3 also has a well-established role in the development of melanocytes during embryogenesis, and has recently been characterized as a molecular switch in the mature melanocyte. Mutations in PAX3 can cause Waardenburg syndrome.