PAX2 Polyclonal antibody

PAX2 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 29307-1-AP

Synonyms

paired box 2, Paired box protein Pax 2, PAX2



Tested Applications

Positive WB detected inmouse cerebellum tissue, rat cerebellum tissue
Positive IHC detected inmouse kidney tissue, human renal cell carcinoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29307-1-AP targets PAX2 in WB, IHC, FC, ELISA applications and shows reactivity with Human, mouse, rat samples.

Tested Reactivity Human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen PAX2 fusion protein Ag30717
Full Name paired box 2
Calculated Molecular Weight 431 aa, 46 kDa
Observed Molecular Weight45 kDa
GenBank Accession NumberBC148710
Gene Symbol PAX2
Gene ID (NCBI) 5076
RRIDAB_2918278
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX2 is a transcription factor, primarily expressed during embryonic development. It has a critical role in the development of the urogenital tract, the eyes, and the CNS. It plays a key role during renal development and to act as an oncogene favoring renal tumor growth. PAX2 was suggested as a sensitive and highly specific marker for renal cell carcinoma (RCC).

Protocols

Product Specific Protocols
WB protocol for PAX2 antibody 29307-1-APDownload protocol
IHC protocol for PAX2 antibody 29307-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Sci Bull (Beijing)

Reprogramming of Ovarian Granulosa Cells by YAP1 Leads to Development of High-Grade Cancer with Mesenchymal Lineage and Serous Features.

Authors - Xiangmin Lv
humanFC

Cells

Human iPSC-Derived Renal Cells Change Their Immunogenic Properties during Maturation: Implications for Regenerative Therapies.

Authors - Bella Rossbach