PAX2 Polyclonal antibody

PAX2 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (1)

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 29307-1-AP

Synonyms

paired box 2, Paired box protein Pax 2, PAX2

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Validation Data Gallery

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  WB analysis using 29307-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29307-1-AP (PAX2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse kidney using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human renal cell carcinoma using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human renal cell carcinoma using 29307-1-AP

  Immunohistochemical analysis of paraffin-embedded human renal cell carcinoma tissue slide using 29307-1-AP (PAX2 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse cerebellum tissue, rat cerebellum tissue
• Positive IHC detected in: mouse kidney tissue, human renal cell carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below
• FC: See 1 publications below

Product Information

29307-1-AP targets PAX2 in WB, FC, IHC, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PAX2 fusion protein Ag30717
• Full Name: paired box 2
• Calculated Molecular Weight: 431 aa, 46 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC148710
• Gene Symbol: PAX2
• Gene ID (NCBI): 5076
• RRID: AB_2918278
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX2 is a transcription factor, primarily expressed during embryonic development. It has a critical role in the development of the urogenital tract, the eyes, and the CNS. It plays a key role during renal development and to act as an oncogene favoring renal tumor growth. PAX2 was suggested as a sensitive and highly specific marker for renal cell carcinoma (RCC).

Publications

Species	Application	Title
human	IHC	Sci Bull (Beijing); Reprogramming of Ovarian Granulosa Cells by YAP1 Leads to Development of High-Grade Cancer with Mesenchymal Lineage and Serous Features.; Authors - Xiangmin Lv
human	FC	Cells; Human iPSC-Derived Renal Cells Change Their Immunogenic Properties during Maturation: Implications for Regenerative Therapies.; Authors - Bella Rossbach