PAX1-Specific Monoclonal antibody

PAX1-Specific Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

5A10D9

Cat no : 60217-1-Ig

Synonyms

HUP48, paired box 1, Paired box protein Pax 1, PAX1

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Validation Data Gallery

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  WB analysis of K-562 using 60217-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 60217-1-Ig (PAX1-Specific Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

60217-1-Ig targets PAX1-Specific in WB, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: mouse
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: paired box 1
• Calculated Molecular Weight: 55 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: NM_006192
• Gene Symbol: PAX1
• Gene ID (NCBI): 5075
• RRID: AB_11183763
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAX1, also named as HUP48, is a transcriptional activator. It may play a role in the formation of segmented structures of the embryo and may play an important role in the normal development of the vertebral column. It is a marker of the sclerotomal compartment. PAX1 was reported that, quantitative measurement of PAX1 hyper-methylation in cervical scrapings is highly sensitive and is more specific than HC2 in detection of cervical cancer. It is expressed by vSMC and pericytes during the earliest phases of vascular wall formation. This antibody is specific to PAX1.

Publications

Species	Application	Title
mouse	IF	Nature; A vertebral skeletal stem cell lineage driving metastasis; Authors - Jun Sun