PARVB Polyclonal antibody

• Featured Product
• KD/KO Validated

PARVB Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

8

Cat no : 14463-1-AP

Synonyms

β-parvin, parvin, beta, CGI-56, CGI 56, Beta-parvin

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Validation Data Gallery

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  WB analysis using 14463-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14463-1-AP (PARVB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 14463-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14463-1-AP (PARVB antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach using 14463-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 14463-1-AP (PARVB antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PARVB antibody (14463-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HeLa using 14463-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using PARVB antibody (14463-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse spleen tissue, mouse heart tissue, rat heart tissue
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 1 publications below

Product Information

14463-1-AP targets PARVB in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: PARVB fusion protein Ag5760
• Full Name: parvin, beta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa and 45 kDa
• GenBank Accession Number: BC046103
• Gene Symbol: PARVB
• Gene ID (NCBI): 29780
• RRID: AB_2158952
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Nature; DNA of neutrophil extracellular traps promotes cancer metastasis via CCDC25.; Authors - Linbin Yang
human	WB	Cell Stem Cell; Integrin-Linked Kinase Mediates Therapeutic Resistance of Quiescent CML Stem Cells to Tyrosine Kinase Inhibitors.; Authors - Katharina Rothe
human	WB	Oncogene; A critical role of integrin-linked kinase, ch-TOG and TACC3 in centrosome clustering in cancer cells.; Authors - Fielding A B AB ; KD Validated
mouse	WB	J Biol Chem; Disruption of the integrin-linked kinase (ILK) pseudokinase domain affects kidney development in mice.; Authors - Nada Bulus
mouse	WB	J Cell Physiol; The Integrin-Mediated ILK-Parvin-αPix Signalling Axis Controls Differentiation in Mammary Epithelial Cells.; Authors - Nicholas Rooney
human	WB	Sci Rep; Integrin-Linked Kinase links Dynactin-1/Dynactin-2 with cortical Integrin receptors to orient the mitotic spindle relative to the substratum.; Authors - Edward James Morris ; KD Validated