PARP4 Recombinant antibody

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  WB analysis using 84001-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84001-1-RR (PARP4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 84001-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84001-1-RR (PARP4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  IHC staining of human stomach cancer using 84001-1-RR

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 84001-1-RR (PARP4 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer(pH9).

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  FC experiment of HeLa using 84001-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug PARP4 Recombinant antibody (84001-1-RR, Clone:240843C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of U-2 OS using 84001-1-RR

  1x10^6 U-2 OS were intracellularly stained with 0.25 ug PARP4 Recombinant antibody (84001-1-RR, Clone:240843C10) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

PARP4 Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 84001-1-RR

Clone No.240843C10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

p193, EC:2.4.2.-, ARTD4, ADPRTL1, ADP-ribosyltransferase diphtheria toxin-like 4

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Tested Applications

• Positive WB detected in: A549 cells, MDA-MB-231 cells, HepG2 cells, U2OS cells, HeLa cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: HeLa cells, U-2 OS

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84001-1-RR targets PARP4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: poly (ADP-ribose) polymerase family, member 4
• Calculated Molecular Weight: 193 kDa
• Observed Molecular Weight: 193 kDa
• GenBank Accession Number: NM_006437
• Gene Symbol: PARP4
• Gene ID (NCBI): 143
• RRID: AB_3671573
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: Q9UKK3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PARP4, also named as ADPRTL1, KIAA0177, PARPL, VPARP and PH5P, catalyzes the reaction: NAD+ + (ADP-D-ribosyl)(n)-acceptor = nicotinamide + (ADP-D-ribosyl)(n+1)-acceptor.